Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium

Igor A. Schepetkin, Liliya N. Kirpotina, Larissa Jakiw, Andrey Ivanovich Khlebnikov, Christie L. Blaskovich, Mark A. Jutila, Mark T. Quinn

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.

Original languageEnglish
Pages (from-to)6754-6766
Number of pages13
JournalJournal of Immunology
Volume183
Issue number10
DOIs
Publication statusPublished - 15 Nov 2009
Externally publishedYes

Fingerprint

Epilobium
Phagocytes
Hydrolyzable Tannins
Pyrogallol
Gallic Acid
Aptitude
Neutrophil Infiltration
Peritoneum
Chemotactic Factors
Chemotaxis
Keratinocytes
oenothein B
Monocytes
Reactive Oxygen Species
Mass Spectrometry
Neutrophils
Macrophages
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium. / Schepetkin, Igor A.; Kirpotina, Liliya N.; Jakiw, Larissa; Khlebnikov, Andrey Ivanovich; Blaskovich, Christie L.; Jutila, Mark A.; Quinn, Mark T.

In: Journal of Immunology, Vol. 183, No. 10, 15.11.2009, p. 6754-6766.

Research output: Contribution to journalArticle

Schepetkin, Igor A. ; Kirpotina, Liliya N. ; Jakiw, Larissa ; Khlebnikov, Andrey Ivanovich ; Blaskovich, Christie L. ; Jutila, Mark A. ; Quinn, Mark T. / Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium. In: Journal of Immunology. 2009 ; Vol. 183, No. 10. pp. 6754-6766.
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