Abstract
Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.
| Original language | English |
|---|---|
| Pages (from-to) | 6754-6766 |
| Number of pages | 13 |
| Journal | Journal of Immunology |
| Volume | 183 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 15 Nov 2009 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium. / Schepetkin, Igor A.; Kirpotina, Liliya N.; Jakiw, Larissa; Khlebnikov, Andrey Ivanovich; Blaskovich, Christie L.; Jutila, Mark A.; Quinn, Mark T.
In: Journal of Immunology, Vol. 183, No. 10, 15.11.2009, p. 6754-6766.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium
AU - Schepetkin, Igor A.
AU - Kirpotina, Liliya N.
AU - Jakiw, Larissa
AU - Khlebnikov, Andrey Ivanovich
AU - Blaskovich, Christie L.
AU - Jutila, Mark A.
AU - Quinn, Mark T.
PY - 2009/11/15
Y1 - 2009/11/15
N2 - Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.
AB - Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.
UR - http://www.scopus.com/inward/record.url?scp=77950822169&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950822169&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.0901827
DO - 10.4049/jimmunol.0901827
M3 - Article
C2 - 19846877
AN - SCOPUS:77950822169
VL - 183
SP - 6754
EP - 6766
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -