Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium

Igor A. Schepetkin, Liliya N. Kirpotina, Larissa Jakiw, Andrey Ivanovich Khlebnikov, Christie L. Blaskovich, Mark A. Jutila, Mark T. Quinn

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.

Original languageEnglish
Pages (from-to)6754-6766
Number of pages13
JournalJournal of Immunology
Issue number10
Publication statusPublished - 15 Nov 2009
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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