TY - JOUR
T1 - Immunomodulatory activity of oenothein B isolated from Epilobium angustifolium
AU - Schepetkin, Igor A.
AU - Kirpotina, Liliya N.
AU - Jakiw, Larissa
AU - Khlebnikov, Andrey Ivanovich
AU - Blaskovich, Christie L.
AU - Jutila, Mark A.
AU - Quinn, Mark T.
PY - 2009/11/15
Y1 - 2009/11/15
N2 - Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.
AB - Epilobium angustifolium has been traditionally used to treat of a number of diseases; however, not much is known regarding its effect on innate immune cells. In this study, we report that extracts of E. angustifolium activated functional responses in neutrophils and monocyte/macrophages. Activity-guided fractionation, followed by mass spectroscopy and NMR analysis, resulted in the identification of oenothein B as the primary component responsible for phagocyte activation. Oenothein B, a dimeric hydrolysable tannin, dosedependently induced a number of phagocyte functions in vitro, including intracellular Ca 2+ flux, production of reactive oxygen species, chemotaxis, NF-κB activation, and proinflammatory cytokine production. Furthermore, oenothein B was active in vivo, inducing keratinocyte chemoattractant production and neutrophil recruitment to the peritoneum after intraperitoneal administration. Biological activity required the full oenothein B structure, as substructures of oenothein B (pyrocatechol, gallic acid, pyrogallol, 3,4-dihydroxybenzoic acid) were all inactive. The ability of oenothein B to modulate phagocyte functions in vitro and in vivo suggests that this compound is responsible for at least part of the therapeutic properties of E. angustifolium extracts.
UR - http://www.scopus.com/inward/record.url?scp=77950822169&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950822169&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.0901827
DO - 10.4049/jimmunol.0901827
M3 - Article
C2 - 19846877
AN - SCOPUS:77950822169
VL - 183
SP - 6754
EP - 6766
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -