Immunohistochemical expression of Bcl-2, p53 and caspase-9 in hypothalamus magnocellular centers of nNOS knockout mice following water deprivation

E. Chernigovskaya, D. Atochin, L. Yamova, P. Huang, M. Glazova

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    We studied the interactions between apoptosis regulator proteins (Bcl-2, p53 and caspase-9) and neuronal nitric oxide in vasopressinergic magnocellular centers of the hypothalamus using neuronal nitric oxide synthase (nNOS) gene knockout mice. nNOS gene deletion resulted in accumulation of Bcl-2, p53 and caspase-9 in the paraventricular (PVN) and supraoptic (SON) nuclei in controls. Dehydration increased the levels of all three apoptosis regulator proteins studied in nuclei of wild type mice. In the hypothalamus magnocellular centers of nNOS knockout mice, however, expression of Bcl-2, p53 and caspase-9 was unchanged after dehydration. The number of magnocellular neurons did not change in the SON and PVN of nNOS deficient mice compared to wild type, and after dehydration, cell death was not observed in either nucleus of wild type or knockout mice despite activation of apoptosis regulator protein expression. Thus, we demonstrated that gene disruption of nNOS prevents activation of Bcl-2, p53 and caspase-9 expression during water deprivation, and that nNOS deficiency did not affect survival of magnocellular neurons of the hypothalamus.

    Original languageEnglish
    Pages (from-to)333-339
    Number of pages7
    JournalBiotechnic and Histochemistry
    Volume86
    Issue number5
    DOIs
    Publication statusPublished - Oct 2011

    Fingerprint

    Water Deprivation
    Nitric Oxide Synthase Type I
    Caspase 9
    Knockout Mice
    Hypothalamus
    Dehydration
    Apoptosis
    Neurons
    Supraoptic Nucleus
    Gene Knockout Techniques
    Proteins
    Gene Deletion
    Nitric Oxide
    Cell Death
    Genes

    Keywords

    • apoptosis regulator proteins
    • hypothalamus
    • magnocellular neurons
    • nitric oxide synthase
    • water deprivation

    ASJC Scopus subject areas

    • Histology
    • Medical Laboratory Technology

    Cite this

    Immunohistochemical expression of Bcl-2, p53 and caspase-9 in hypothalamus magnocellular centers of nNOS knockout mice following water deprivation. / Chernigovskaya, E.; Atochin, D.; Yamova, L.; Huang, P.; Glazova, M.

    In: Biotechnic and Histochemistry, Vol. 86, No. 5, 10.2011, p. 333-339.

    Research output: Contribution to journalArticle

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