Abstract
Accumulating evidence shows that S-nitrosothiols, formed by the addition of nitric oxide (NO) to a cysteine thiol, S-nitrosylation, are involved in basal cellular regulation. It has been proposed that SNO formation/removal may be disrupted in a variety of pathophysiological conditions. Two types of methodology are presently available to identify specific S-nitrosylated proteins: (1) derivatization and (2) post-purification chemical detection. Neither of these techniques allows for in situ visualization of SNOs. Recently, we demonstrated that an antibody generated to the SNO moiety could be used to detect SNO formation from each of three isoforms of NOS by immunohistochemistry. This chapter details the immunohistochemical methodology used to detect SNOs in situ, offering a potentially powerful alternative for detection of SNO within tissue sections.
| Original language | English |
|---|---|
| Pages (from-to) | 167-172 |
| Number of pages | 6 |
| Journal | Methods in Molecular Biology |
| Volume | 279 |
| Publication status | Published - 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Immunohistochemical detection of S-nitrosylated proteins. / Gow, Andrew J.; Davis, Christiana W.; Munson, David; Ischiropoulos, Harry.
In: Methods in Molecular Biology, Vol. 279, 2004, p. 167-172.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical detection of S-nitrosylated proteins.
AU - Gow, Andrew J.
AU - Davis, Christiana W.
AU - Munson, David
AU - Ischiropoulos, Harry
PY - 2004
Y1 - 2004
N2 - Accumulating evidence shows that S-nitrosothiols, formed by the addition of nitric oxide (NO) to a cysteine thiol, S-nitrosylation, are involved in basal cellular regulation. It has been proposed that SNO formation/removal may be disrupted in a variety of pathophysiological conditions. Two types of methodology are presently available to identify specific S-nitrosylated proteins: (1) derivatization and (2) post-purification chemical detection. Neither of these techniques allows for in situ visualization of SNOs. Recently, we demonstrated that an antibody generated to the SNO moiety could be used to detect SNO formation from each of three isoforms of NOS by immunohistochemistry. This chapter details the immunohistochemical methodology used to detect SNOs in situ, offering a potentially powerful alternative for detection of SNO within tissue sections.
AB - Accumulating evidence shows that S-nitrosothiols, formed by the addition of nitric oxide (NO) to a cysteine thiol, S-nitrosylation, are involved in basal cellular regulation. It has been proposed that SNO formation/removal may be disrupted in a variety of pathophysiological conditions. Two types of methodology are presently available to identify specific S-nitrosylated proteins: (1) derivatization and (2) post-purification chemical detection. Neither of these techniques allows for in situ visualization of SNOs. Recently, we demonstrated that an antibody generated to the SNO moiety could be used to detect SNO formation from each of three isoforms of NOS by immunohistochemistry. This chapter details the immunohistochemical methodology used to detect SNOs in situ, offering a potentially powerful alternative for detection of SNO within tissue sections.
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UR - http://www.scopus.com/inward/citedby.url?scp=4444335180&partnerID=8YFLogxK
M3 - Article
C2 - 15199244
AN - SCOPUS:4444335180
VL - 279
SP - 167
EP - 172
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -