Abstract
The role of S-nitrosylation in cellular signaling has been clearly demonstrated. There a number of mechanisms whereby this post-translational modification can occur and the number of protein targets continue to expand. The need to be able to monitor when this important signaling process occurs within cells is increasingly important. Previously we have identified immunohistochemistry approaches effective for monitoring S-nitrosylation within fixed tissue. Within this paper we show how these techniques can be adapted to use in a cell culture system using immunofluorescence. We have used this protocol to detect S-nitrosoprotein formation within LPS stimulated microglial cells using both transformed and primary cultured cells.
| Original language | English |
|---|---|
| Pages (from-to) | 161-164 |
| Number of pages | 4 |
| Journal | Methods |
| Volume | 62 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Aug 2013 |
| Externally published | Yes |
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Keywords
- Immunofluorescence
- Microglia
- Nitric oxide
- Nitrosothiol
- Thiol
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Immunofluorescent detection of S-nitrosoproteins in cell culture. / Rossi-George, Alba; Gow, Andrew J.
In: Methods, Vol. 62, No. 2, 01.08.2013, p. 161-164.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunofluorescent detection of S-nitrosoproteins in cell culture
AU - Rossi-George, Alba
AU - Gow, Andrew J.
PY - 2013/8/1
Y1 - 2013/8/1
N2 - The role of S-nitrosylation in cellular signaling has been clearly demonstrated. There a number of mechanisms whereby this post-translational modification can occur and the number of protein targets continue to expand. The need to be able to monitor when this important signaling process occurs within cells is increasingly important. Previously we have identified immunohistochemistry approaches effective for monitoring S-nitrosylation within fixed tissue. Within this paper we show how these techniques can be adapted to use in a cell culture system using immunofluorescence. We have used this protocol to detect S-nitrosoprotein formation within LPS stimulated microglial cells using both transformed and primary cultured cells.
AB - The role of S-nitrosylation in cellular signaling has been clearly demonstrated. There a number of mechanisms whereby this post-translational modification can occur and the number of protein targets continue to expand. The need to be able to monitor when this important signaling process occurs within cells is increasingly important. Previously we have identified immunohistochemistry approaches effective for monitoring S-nitrosylation within fixed tissue. Within this paper we show how these techniques can be adapted to use in a cell culture system using immunofluorescence. We have used this protocol to detect S-nitrosoprotein formation within LPS stimulated microglial cells using both transformed and primary cultured cells.
KW - Immunofluorescence
KW - Microglia
KW - Nitric oxide
KW - Nitrosothiol
KW - Thiol
UR - http://www.scopus.com/inward/record.url?scp=84882677861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882677861&partnerID=8YFLogxK
U2 - 10.1016/j.ymeth.2013.05.022
DO - 10.1016/j.ymeth.2013.05.022
M3 - Article
C2 - 23748109
AN - SCOPUS:84882677861
VL - 62
SP - 161
EP - 164
JO - Methods
JF - Methods
SN - 1046-2023
IS - 2
ER -