Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles

A. M. Demin, M. S. Valova, A. G. Pershina, V. P. Krasnov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nano particles (MNPs), preliminarily functionalized with 3-aminopropylsilane and N-(phosphonomethyl) iminodiacetic acid (PMIDA), was studied. Protein binding to MNPs depending on the nature of the surface functional groups, the medium, and the presence of a coupling agent, namely, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC), was studied using fluorimetry, UV and IR spectroscopy. It was shown that protein immobilization can occur by both non-covalent and covalent binding with surface groups of MNPs. The best results (up to 150 μg of TagGFP2 per 1 mg of MNPs) were obtained for MNPs preliminarily functionalized with PMIDA and using EDC. The results of this study can be used for the design of imaging agents having both magnetic and optical properties to be applied in biomedicine.

Original languageEnglish
Pages (from-to)1088-1095
Number of pages8
JournalRussian Chemical Bulletin
Volume68
Issue number5
DOIs
Publication statusPublished - 1 May 2019

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Ethyldimethylaminopropyl Carbodiimide
Nanoparticles
Coupling agents
Ultraviolet spectroscopy
Functional groups
Infrared spectroscopy
Magnetic properties
Proteins
Optical properties
Imaging techniques
iminodiacetic acid
Protein Binding
aminopropylsilane

Keywords

  • fluorimetry
  • magnetic nanoparticles
  • TagGFP2
  • UV spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Immobilization of fluorescent protein TagGFP2 on Fe3O4-based magnetic nanoparticles. / Demin, A. M.; Valova, M. S.; Pershina, A. G.; Krasnov, V. P.

In: Russian Chemical Bulletin, Vol. 68, No. 5, 01.05.2019, p. 1088-1095.

Research output: Contribution to journalArticle

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