Immobilization of bovine serum albumin onto porous poly(vinylpyrrolidone)- modified silicas

Alexander S. Timin, Alexey V. Solomonov, Irek I. Musabirov, Semen N. Sergeev, Sergey P. Ivanov, Evgeniy V. Rumyantsev, Alexander Goncharenko

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Grafting with poly(N-vinyl-2-pyrrolidone) (PVP) was used to functionalize silica. The functionalized silicas were characterized by scanning electron microscopy, nitrogen adsorption, energy-dispersive X-ray analysis, Fourier transform spectroscopy, and thermogravimetric analysis. The Barrett-Joyner- Halenda analysis confirmed that PVP-modified silica samples have a mesoporous structure. Bovine serum albumin (BSA) was immobilized onto the surface of PVP-modified silica systems through physical adsorption. Also it was shown that the adsorption capacity of the tested samples depends on the quantity of PVP inside the silica matrix, which confirmed the formation of a specific complex between BSA and PVP. In addition, the effects of pH, time, and concentration on BSA adsorption were investigated. The maximum BSA adsorption capacity was 71.54 ± 2.0 mg/g. The desorption and reusability of all prepared samples were studied as well. PVP-modified silicas are capable of holding more BSA compared to nonmodified silica. These characteristics indicate that PVP-modified silicas have great potential for efficient protein encapsulation.

Original languageEnglish
Pages (from-to)13699-13710
Number of pages12
JournalIndustrial and Engineering Chemistry Research
Volume53
Issue number35
DOIs
Publication statusPublished - 3 Sep 2014
Externally publishedYes

Fingerprint

Bovine Serum Albumin
Silicon Dioxide
Silica
Adsorption
Energy dispersive X ray analysis
Reusability
Encapsulation
Thermogravimetric analysis
Desorption
Fourier transforms
Nitrogen
Spectroscopy
Proteins
Scanning electron microscopy

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)
  • Industrial and Manufacturing Engineering

Cite this

Timin, A. S., Solomonov, A. V., Musabirov, I. I., Sergeev, S. N., Ivanov, S. P., Rumyantsev, E. V., & Goncharenko, A. (2014). Immobilization of bovine serum albumin onto porous poly(vinylpyrrolidone)- modified silicas. Industrial and Engineering Chemistry Research, 53(35), 13699-13710. https://doi.org/10.1021/ie501915f

Immobilization of bovine serum albumin onto porous poly(vinylpyrrolidone)- modified silicas. / Timin, Alexander S.; Solomonov, Alexey V.; Musabirov, Irek I.; Sergeev, Semen N.; Ivanov, Sergey P.; Rumyantsev, Evgeniy V.; Goncharenko, Alexander.

In: Industrial and Engineering Chemistry Research, Vol. 53, No. 35, 03.09.2014, p. 13699-13710.

Research output: Contribution to journalArticle

Timin, AS, Solomonov, AV, Musabirov, II, Sergeev, SN, Ivanov, SP, Rumyantsev, EV & Goncharenko, A 2014, 'Immobilization of bovine serum albumin onto porous poly(vinylpyrrolidone)- modified silicas', Industrial and Engineering Chemistry Research, vol. 53, no. 35, pp. 13699-13710. https://doi.org/10.1021/ie501915f
Timin, Alexander S. ; Solomonov, Alexey V. ; Musabirov, Irek I. ; Sergeev, Semen N. ; Ivanov, Sergey P. ; Rumyantsev, Evgeniy V. ; Goncharenko, Alexander. / Immobilization of bovine serum albumin onto porous poly(vinylpyrrolidone)- modified silicas. In: Industrial and Engineering Chemistry Research. 2014 ; Vol. 53, No. 35. pp. 13699-13710.
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