Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis

Ekaterina A. Perina, Vladimir V. Ivanov, Alexandra G. Pershina, Natalya A. Perekucha, Anna N. Dzyuman, Ilya P. Kaminskii, Irina V. Saltykova, Alexey E. Sazonov, Ludmila M. Ogorodova

Research output: Contribution to journalArticle

Abstract

Although data on oxidative stress during liver fluke infection have been previously presented, a comprehensive study of the glutathione system that plays a crucial role in scavenging of reactive oxygen species (ROS) and detoxification of primary and secondary oxidation products has not been addressed yet. In the present study, the hepatic glutathione system was investigated in a hamster model of experimental opisthorchiasis infection. It was shown that chronic oxidative stress in an Opisthorchis felineus infected liver, evidenced by abundant hydroperoxide accumulation, leads to strong imbalance in the hepatic glutathione system, namely the depletion of reduced form of glutathione (GSH), lowering of the GSH/GSSG ratio, and a decrease in the glutathione peroxidase and glyoxalase 1 activity. O. felineus infection provokes hepatocellular damage that results in the progression of liver fibrosis, accompanied by an increase in collagen deposition in the hepatic tissue. Modulation of hepatic GSH levels in the O. felineus infected liver through N-acetylcysteine (NAC) or L-buthionine-S, R-sulfoxinine (BSO) treatments lead to changes in expression and activity of glutathione S-transferase and glyoxalase I as well as markedly decreases or increases collagen content in the O. felineus infected liver and the severity of liver fibrosis, respectively. Thus, the glutathione system can be considered as a target for liver protection from O. felineus-induced injury.

Original languageEnglish
Pages (from-to)41-48
Number of pages8
JournalActa Tropica
Volume192
DOIs
Publication statusPublished - 1 Apr 2019

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Opisthorchis
liver cirrhosis
Glutathione
glutathione
Fibrosis
liver
Liver
Liver Cirrhosis
collagen
Oxidative Stress
oxidative stress
Collagen
Infection
Lactoylglutathione Lyase
Opisthorchiasis
trematode infections
liver flukes
Fasciola hepatica
acetylcysteine
Glutathione Disulfide

Keywords

  • Glutathione system
  • L-buthionine-S
  • Liver fibrosis
  • N-acetylcysteine
  • Opisthorchis felineus
  • Oxidative stress
  • R-sulfoxinine

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

Cite this

Perina, E. A., Ivanov, V. V., Pershina, A. G., Perekucha, N. A., Dzyuman, A. N., Kaminskii, I. P., ... Ogorodova, L. M. (2019). Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis. Acta Tropica, 192, 41-48. https://doi.org/10.1016/j.actatropica.2019.01.017

Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis. / Perina, Ekaterina A.; Ivanov, Vladimir V.; Pershina, Alexandra G.; Perekucha, Natalya A.; Dzyuman, Anna N.; Kaminskii, Ilya P.; Saltykova, Irina V.; Sazonov, Alexey E.; Ogorodova, Ludmila M.

In: Acta Tropica, Vol. 192, 01.04.2019, p. 41-48.

Research output: Contribution to journalArticle

Perina, EA, Ivanov, VV, Pershina, AG, Perekucha, NA, Dzyuman, AN, Kaminskii, IP, Saltykova, IV, Sazonov, AE & Ogorodova, LM 2019, 'Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis', Acta Tropica, vol. 192, pp. 41-48. https://doi.org/10.1016/j.actatropica.2019.01.017
Perina, Ekaterina A. ; Ivanov, Vladimir V. ; Pershina, Alexandra G. ; Perekucha, Natalya A. ; Dzyuman, Anna N. ; Kaminskii, Ilya P. ; Saltykova, Irina V. ; Sazonov, Alexey E. ; Ogorodova, Ludmila M. / Imbalance in the glutathione system in Opisthorchis felineus infected liver promotes hepatic fibrosis. In: Acta Tropica. 2019 ; Vol. 192. pp. 41-48.
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