Hypercholesterolemia worsens the blood flow deficit in focal cerebral ischemia in the absence of atherosclerosis

Kyoung Shin Hwa, James A Lapointe Li, Dmitriy N. Atochin, Michael A. Moskowitz, Paul L. Huang, Cenk Ayata

Research output: Contribution to journalArticle

Abstract

Background and aims: Hypercholesterolemia may increase stroke risk by accelerating atherosclerosis and narrowing luminal diameter in cerebral vessels as well as disrupting vascular endothelial and smooth muscle function. We tested the hypothesis that that hypercholesterolemia impairs cerebrovascular function and worsens cerebral blood flow (CBF) deficit and tissue outcome after focal ischemia even in the absence of hemodynamically significant vessel narrowing. Methods: We tested this hypothesis in ApoE knockout (KO) mice using high spatiotemporal resolution laser speckle flowmetry through intact skull noninvasively, and studied the hemodynamic impact of hypercholesterolemia on the evolution of CBF deficit in focal cerebral ischemia. Wild type (WT, C57BL/6), ApoE KO or ApoE/eNOS double KO mice were fed high-fat quot;westernquot; diet for 4, 8, or 10 weeks starting at 4 weeks of age. Histopathological examination of extracranial arteries in 14 week-old ApoE KO showed only minimal atherosclerotic changes such as fatty streaks with subendothelial foam cells. Intracranial arteries did not develop atherosclerotic lesions even after 12 months, despite severe extracranial atherosclerosis. Results: Mean arterial blood pressure was higher in ApoE KO compared to WT in all age groups (94+/-2 vs. 85+/-2 mmHg, respectively; n=17 each, p

Original languageEnglish
JournalJournal of Cerebral Blood Flow and Metabolism
Volume27
Issue numberSUPPL. 1
Publication statusPublished - 13 Nov 2007
Externally publishedYes

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Apolipoproteins E
Hypercholesterolemia
Brain Ischemia
Atherosclerosis
Cerebrovascular Circulation
Knockout Mice
Arterial Pressure
Arteries
Foam Cells
Rheology
Vascular Smooth Muscle
Skull
Lasers
Ischemia
Age Groups
Hemodynamics
Stroke
Fats
Diet

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Hypercholesterolemia worsens the blood flow deficit in focal cerebral ischemia in the absence of atherosclerosis. / Hwa, Kyoung Shin; Li, James A Lapointe; Atochin, Dmitriy N.; Moskowitz, Michael A.; Huang, Paul L.; Ayata, Cenk.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 27, No. SUPPL. 1, 13.11.2007.

Research output: Contribution to journalArticle

Hwa, Kyoung Shin ; Li, James A Lapointe ; Atochin, Dmitriy N. ; Moskowitz, Michael A. ; Huang, Paul L. ; Ayata, Cenk. / Hypercholesterolemia worsens the blood flow deficit in focal cerebral ischemia in the absence of atherosclerosis. In: Journal of Cerebral Blood Flow and Metabolism. 2007 ; Vol. 27, No. SUPPL. 1.
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