TY - JOUR
T1 - Hybrid capsules as efficient non-viral vector for gene delivery into cells
AU - Zyuzin, M. V.
AU - Timin, A. S.
N1 - Funding Information:
Synthesis of capsules was supported by the Russian Science Foundation (grant No 17-19-01637), cell experiments were supported by the Russian Foundation for Basic Research (grant No 18-015-00100). MVZ thanks the President’s Scholarship SP-1576.2018.4.
Publisher Copyright:
© 2018 Institute of Physics Publishing.All Rights Reserved.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018
Y1 - 2018
N2 - Advances in the field of nanotechnology make it possible to employ nano-and microparticles as delivery carriers of genetic materials. In this study, hollow capsules of different composition were used as non-viral vectors to deliver plasmids into human cervical carcinoma cells (HeLa). Plasmids were encoded to express green fluorescent protein (eGFP) inside cells. Hybrid capsules were produced from degradable polyelectrolytes with an outer silica layer. Capsules were characterized with scanning electron and bright field microscopy. Successful cell transfection was verified with the confocal laser scanning microscope (CLSM).
AB - Advances in the field of nanotechnology make it possible to employ nano-and microparticles as delivery carriers of genetic materials. In this study, hollow capsules of different composition were used as non-viral vectors to deliver plasmids into human cervical carcinoma cells (HeLa). Plasmids were encoded to express green fluorescent protein (eGFP) inside cells. Hybrid capsules were produced from degradable polyelectrolytes with an outer silica layer. Capsules were characterized with scanning electron and bright field microscopy. Successful cell transfection was verified with the confocal laser scanning microscope (CLSM).
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U2 - 10.1088/1742-6596/1092/1/012182
DO - 10.1088/1742-6596/1092/1/012182
M3 - Conference article
AN - SCOPUS:85056234882
VL - 1092
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
SN - 1742-6588
M1 - 012182
T2 - 3rd International Conference on Metamaterials and Nanophotonics, METANANO 2018
Y2 - 17 September 2018 through 21 September 2018
ER -