HPLC determination of nitrated polycyclic aromatic hydrocarbons after their reduction to amino derivatives

Jiri Brichac, Jiri Zima, Jiri Barek

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12 Citations (Scopus)


High-performance liquid chromatography with fluorescence detection (HPLC-FD) was used for the determination of 1-nitropyrene, 2-nitrofluorene, and 6-nitrochrysene after their reduction to amino derivatives. As a reducing agent, titanium(III) complexed with citrate in a hydrochloric acid medium was used. Conditions for 1-nitropyrene, 2-nitrofluorene, and 6-nitrochrysene reduction, and for separation of corresponding amino derivatives were found. The limit of detection was 68 pg for 2-nitrofluorene, 82 pg for 2-nitropyrene, and 629 pg for 6-nitrochrysene. The applicability of this method for the determination of 1-nitropyrene in water samples was verified.

Original languageEnglish
Pages (from-to)2379-2392
Number of pages14
JournalAnalytical Letters
Issue number11
Publication statusPublished - 12 Oct 2004
Externally publishedYes



  • Fluorescence detection
  • HPLC
  • Nitrated polycyclic aromatic hydrocarbons (NPAH)
  • Reduction by titanium(III)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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