HPLC determination of nitrated polycyclic aromatic hydrocarbons after their reduction to amino derivatives

Jiri Brichac, Jiri Zima, Jiri Barek

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

High-performance liquid chromatography with fluorescence detection (HPLC-FD) was used for the determination of 1-nitropyrene, 2-nitrofluorene, and 6-nitrochrysene after their reduction to amino derivatives. As a reducing agent, titanium(III) complexed with citrate in a hydrochloric acid medium was used. Conditions for 1-nitropyrene, 2-nitrofluorene, and 6-nitrochrysene reduction, and for separation of corresponding amino derivatives were found. The limit of detection was 68 pg for 2-nitrofluorene, 82 pg for 2-nitropyrene, and 629 pg for 6-nitrochrysene. The applicability of this method for the determination of 1-nitropyrene in water samples was verified.

Original languageEnglish
Pages (from-to)2379-2392
Number of pages14
JournalAnalytical Letters
Volume37
Issue number11
DOIs
Publication statusPublished - 12 Oct 2004
Externally publishedYes

    Fingerprint

Keywords

  • Fluorescence detection
  • HPLC
  • Nitrated polycyclic aromatic hydrocarbons (NPAH)
  • Reduction by titanium(III)

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

Cite this