Holographic diagnostics of biological microparticles

Victor V. Dyomin, Vladimir V. Sokolov

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)


Problem of studies of biological microojects is actual one for ecology, medicine, biology. Holographic techniques are useful to solve the problem. The above microojects are transparent or semitransparent ones in a visible light rather often. The case of an optically soft particle, (that is of a particle whose substance has the refractive index close to that of the surrounding medium) is quite probable in biological water suspensions. Some peculiarities of holographing optically soft microparticles are analyzed in this paper. We propose a technique to calculate a light intensity distribution in the plane of a hologram and in the plane of a holographic image of a particle of an arbitrary shape at an arbitrary distance from the latter plane. The efficiency of the approach proposed is demonstrated by calculational results obtained analytically for some simple cases. In a more complicated cases the technique can make a basis for numerical computations. The method of determining of refractive index of transparent and semitransparent microparticles is proposed. We also present in this paper some experimental results on holographic detection of the water drops and such optically soft particles as ovums of helmints in human jaundice.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsDaniel L. Farkas, Robert C. Leif, Alexander V. Priezzhev, Toshimitsu Asakura, Bruce J. Tromberg
Number of pages9
Publication statusPublished - 1 Jan 1996
Externally publishedYes
EventOptical Diagnostics of Living Cells and Biofluids - San Jose, CA, USA
Duration: 28 Jan 19961 Feb 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering


ConferenceOptical Diagnostics of Living Cells and Biofluids
CitySan Jose, CA, USA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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