Abstract
Problem of studies of biological microojects is actual one for ecology, medicine, biology. Holographic techniques are useful to solve the problem. The above microojects are transparent or semitransparent ones in a visible light rather often. The case of an optically soft particle, (that is of a particle whose substance has the refractive index close to that of the surrounding medium) is quite probable in biological water suspensions. Some peculiarities of holographing optically soft microparticles are analyzed in this paper. We propose a technique to calculate a light intensity distribution in the plane of a hologram and in the plane of a holographic image of a particle of an arbitrary shape at an arbitrary distance from the latter plane. The efficiency of the approach proposed is demonstrated by calculational results obtained analytically for some simple cases. In a more complicated cases the technique can make a basis for numerical computations. The method of determining of refractive index of transparent and semitransparent microparticles is proposed. We also present in this paper some experimental results on holographic detection of the water drops and such optically soft particles as ovums of helmints in human jaundice.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | Daniel L. Farkas, Robert C. Leif, Alexander V. Priezzhev, Toshimitsu Asakura, Bruce J. Tromberg |
| Pages | 534-542 |
| Number of pages | 9 |
| Publication status | Published - 1 Jan 1996 |
| Externally published | Yes |
| Event | Optical Diagnostics of Living Cells and Biofluids - San Jose, CA, USA Duration: 28 Jan 1996 → 1 Feb 1996 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 2678 |
Conference
| Conference | Optical Diagnostics of Living Cells and Biofluids |
|---|---|
| City | San Jose, CA, USA |
| Period | 28.1.96 → 1.2.96 |
Fingerprint
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering
Cite this
Holographic diagnostics of biological microparticles. / Dyomin, Victor V.; Sokolov, Vladimir V.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / Daniel L. Farkas; Robert C. Leif; Alexander V. Priezzhev; Toshimitsu Asakura; Bruce J. Tromberg. 1996. p. 534-542 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2678).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Holographic diagnostics of biological microparticles
AU - Dyomin, Victor V.
AU - Sokolov, Vladimir V.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - Problem of studies of biological microojects is actual one for ecology, medicine, biology. Holographic techniques are useful to solve the problem. The above microojects are transparent or semitransparent ones in a visible light rather often. The case of an optically soft particle, (that is of a particle whose substance has the refractive index close to that of the surrounding medium) is quite probable in biological water suspensions. Some peculiarities of holographing optically soft microparticles are analyzed in this paper. We propose a technique to calculate a light intensity distribution in the plane of a hologram and in the plane of a holographic image of a particle of an arbitrary shape at an arbitrary distance from the latter plane. The efficiency of the approach proposed is demonstrated by calculational results obtained analytically for some simple cases. In a more complicated cases the technique can make a basis for numerical computations. The method of determining of refractive index of transparent and semitransparent microparticles is proposed. We also present in this paper some experimental results on holographic detection of the water drops and such optically soft particles as ovums of helmints in human jaundice.
AB - Problem of studies of biological microojects is actual one for ecology, medicine, biology. Holographic techniques are useful to solve the problem. The above microojects are transparent or semitransparent ones in a visible light rather often. The case of an optically soft particle, (that is of a particle whose substance has the refractive index close to that of the surrounding medium) is quite probable in biological water suspensions. Some peculiarities of holographing optically soft microparticles are analyzed in this paper. We propose a technique to calculate a light intensity distribution in the plane of a hologram and in the plane of a holographic image of a particle of an arbitrary shape at an arbitrary distance from the latter plane. The efficiency of the approach proposed is demonstrated by calculational results obtained analytically for some simple cases. In a more complicated cases the technique can make a basis for numerical computations. The method of determining of refractive index of transparent and semitransparent microparticles is proposed. We also present in this paper some experimental results on holographic detection of the water drops and such optically soft particles as ovums of helmints in human jaundice.
UR - http://www.scopus.com/inward/record.url?scp=0029728662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029728662&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0029728662
SN - 0819420522
SN - 9780819420527
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 534
EP - 542
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Farkas, Daniel L.
A2 - Leif, Robert C.
A2 - Priezzhev, Alexander V.
A2 - Asakura, Toshimitsu
A2 - Tromberg, Bruce J.
ER -