Histological study of subcutaneous fat at NIR laser treatment of the rat skin in vivo

I. Yu Yanina, Yu I. Svenskaya, N. A. Navolokin, O. V. Matveeva, A. B. Bucharskaya, G. N. Maslyakova, D. A. Gorin, G. B. Sukhorukov, V. V. Tuchin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The goal of this work is to quantify impact of in vivo photochemical treatment using indocyanine green (ICG) or encapsulated ICG and NIR laser irradiation through skin of rat with obesity by the follow up tissue sampling and histochemistry. After 1 hour elapsed since 1-min light exposure samples of rat skin with subcutaneous tissue of thickness of 1.5-2.5 mm were taken by surgery from rats within marked 4-zones of the skin site. For hematoxylin-eosin histological examination of excised tissue samples, fixation was carried out by 10%-formaldehyde solution. For ICG and encapsulated ICG subcutaneous injection and subsequent 1-min diode laser irradiation with power density of 8 W/cm2, different necrotic regions with lipolysis of subcutaneous fat were observed. The obtained data can be used for safe layer-by-layer laser treatment of obesity and cellulite.

Original languageEnglish
Title of host publicationMedical Laser Applications and Laser-Tissue Interactions VII
PublisherSPIE
Volume9542
ISBN (Electronic)9781628417074
DOIs
Publication statusPublished - 2015
Externally publishedYes
EventMedical Laser Applications and Laser Tissue Interactions VII - Munich, Germany
Duration: 21 Jun 201523 Jun 2015

Conference

ConferenceMedical Laser Applications and Laser Tissue Interactions VII
CountryGermany
CityMunich
Period21.6.1523.6.15

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Keywords

  • Adipose tissue
  • Histological analysis
  • Laser irradiation
  • Necrosis
  • Photodynamic action

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Yanina, I. Y., Svenskaya, Y. I., Navolokin, N. A., Matveeva, O. V., Bucharskaya, A. B., Maslyakova, G. N., Gorin, D. A., Sukhorukov, G. B., & Tuchin, V. V. (2015). Histological study of subcutaneous fat at NIR laser treatment of the rat skin in vivo. In Medical Laser Applications and Laser-Tissue Interactions VII (Vol. 9542). [954216] SPIE. https://doi.org/10.1117/12.2183722