Abstract
Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.
Original language | English |
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Pages (from-to) | 923-929 |
Number of pages | 7 |
Journal | Nuclear Medicine and Biology |
Volume | 26 |
Issue number | 8 |
DOIs | |
Publication status | Published - Nov 1999 |
Externally published | Yes |
Keywords
- Br
- A33
- CAT-labeling
- Monoclonal antibodies
- PET
- Radiobromination
ASJC Scopus subject areas
- Molecular Medicine
- Radiology Nuclear Medicine and imaging
- Cancer Research