High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T

Johanna Sundin, Vladimir Tolmachev, Jacek Koziorowski, Jörgen Carlsson, Hans Lundqvist, Sydney Welt, Steven Larson, Anders Sundin

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

Original languageEnglish
Pages (from-to)923-929
Number of pages7
JournalNuclear Medicine and Biology
Volume26
Issue number8
DOIs
Publication statusPublished - Nov 1999
Externally publishedYes

Keywords

  • Br
  • A33
  • CAT-labeling
  • Monoclonal antibodies
  • PET
  • Radiobromination

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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