High yield direct <sup>76</sup>Br-bromination of monoclonal antibodies using chloramine-T

Abstract

Monoclonal antibody (MAb) A33 was labeled with the positron emitter ⁷⁶Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of ⁷⁶Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were ⁷⁶Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with ⁷⁶Br for the purpose of whole-body tumour imaging by positron emission tomography.

Original language	English
Pages (from-to)	923-929
Number of pages	7
Journal	Nuclear Medicine and Biology
Volume	26
Issue number	8
DOIs	https://doi.org/10.1016/S0969-8051(99)00076-1
Publication status	Published - Nov 1999
Externally published	Yes

Keywords

Br
A33
CAT-labeling
Monoclonal antibodies
PET
Radiobromination

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Cancer Research

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10.1016/S0969-8051(99)00076-1

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@article{90dc40506cc54295b7b6dab305037bdc,

title = "High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T",

abstract = "Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.",

keywords = "Br, A33, CAT-labeling, Monoclonal antibodies, PET, Radiobromination",

author = "Johanna Sundin and Vladimir Tolmachev and Jacek Koziorowski and J{\"o}rgen Carlsson and Hans Lundqvist and Sydney Welt and Steven Larson and Anders Sundin",

note = "Funding Information: The authors thank the staff at the Uppsala University PET Centre for their assistance with the radiobromine production. We also thank the The Svedberg Laboratory for allowing us to use their facilities. This work was supported by the Swedish Cancer Foundation (grant 2971-B97-08XAB). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1999",

month = nov,

doi = "10.1016/S0969-8051(99)00076-1",

language = "English",

volume = "26",

pages = "923--929",

journal = "Nuclear Medicine and Biology",

issn = "0969-8051",

publisher = "Elsevier Inc.",

number = "8",

}

TY - JOUR

T1 - High yield direct 76Br-bromination of monoclonal antibodies using chloramine-T

AU - Sundin, Johanna

AU - Tolmachev, Vladimir

AU - Koziorowski, Jacek

AU - Carlsson, Jörgen

AU - Lundqvist, Hans

AU - Welt, Sydney

AU - Larson, Steven

AU - Sundin, Anders

N1 - Funding Information: The authors thank the staff at the Uppsala University PET Centre for their assistance with the radiobromine production. We also thank the The Svedberg Laboratory for allowing us to use their facilities. This work was supported by the Swedish Cancer Foundation (grant 2971-B97-08XAB). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1999/11

Y1 - 1999/11

N2 - Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

AB - Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean ± max error) of 77 ± 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean ± max error) of 75 ± 3%, 63 ± 4%, and 73 ± 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

KW - Br

KW - A33

KW - CAT-labeling

KW - Monoclonal antibodies

KW - PET

KW - Radiobromination

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