High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels

Svetlana Gorodzha, Timothy E L Douglas, Sangram K. Samal, Rainer Detsch, Katarzyna Cholewa-Kowalska, Kevin Braeckmans, Aldo R. Boccaccini, Andre G. Skirtach, Venera Weinhardt, Tilo Baumbach, Maria A. Surmeneva, Roman A. Surmenev

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Enrichment of hydrogels with inorganic particles improves their suitability for bone regeneration by enhancing their mechanical properties, mineralizability, and bioactivity as well as adhesion, proliferation, and differentiation of bone-forming cells, while maintaining injectability. Low aggregation and homogeneous distribution maximize particle surface area, promoting mineralization, cell-particle interactions, and homogenous tissue regeneration. Hence, determination of the size and distribution of particles/particle agglomerates in the hydrogel is desirable. Commonly used techniques have drawbacks. High-resolution techniques (e.g., SEM) require drying. Distribution in the dry state is not representative of the wet state. Techniques in the wet state (histology, μCT) are of lower resolution. Here, self-gelling, injectable composites of Gellan Gum (GG) hydrogel and two different types of sol-gel-derived bioactive glass (bioglass) particles were analyzed in the wet state using Synchrotron X-ray radiation, enabling high-resolution determination of particle size and spatial distribution. The lower detection limit volume was 9 × 10-5 mm3. Bioglass particle suspensions were also studied using zeta potential measurements and Coulter analysis. Aggregation of bioglass particles in the GG hydrogels occurred and aggregate distribution was inhomogeneous. Bioglass promoted attachment of rat mesenchymal stem cells (rMSC) and mineralization.

Original languageEnglish
JournalJournal of Biomedical Materials Research - Part A
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Bioactive glass
Hydrogels
Hydrogel
X ray analysis
Synchrotrons
Particles (particulate matter)
Bone
Agglomeration
Tissue regeneration
Histology
Particle interactions
Zeta potential
Bioactivity
Stem cells
Particle size analysis
Spatial distribution
Sol-gels
Rats
Suspensions
Drying

Keywords

  • Bioglass
  • Composite
  • Hydrogel
  • Particle
  • X-ray

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels. / Gorodzha, Svetlana; Douglas, Timothy E L; Samal, Sangram K.; Detsch, Rainer; Cholewa-Kowalska, Katarzyna; Braeckmans, Kevin; Boccaccini, Aldo R.; Skirtach, Andre G.; Weinhardt, Venera; Baumbach, Tilo; Surmeneva, Maria A.; Surmenev, Roman A.

In: Journal of Biomedical Materials Research - Part A, 2016.

Research output: Contribution to journalArticle

Gorodzha, S, Douglas, TEL, Samal, SK, Detsch, R, Cholewa-Kowalska, K, Braeckmans, K, Boccaccini, AR, Skirtach, AG, Weinhardt, V, Baumbach, T, Surmeneva, MA & Surmenev, RA 2016, 'High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels', Journal of Biomedical Materials Research - Part A. https://doi.org/10.1002/jbm.a.35642
Gorodzha, Svetlana ; Douglas, Timothy E L ; Samal, Sangram K. ; Detsch, Rainer ; Cholewa-Kowalska, Katarzyna ; Braeckmans, Kevin ; Boccaccini, Aldo R. ; Skirtach, Andre G. ; Weinhardt, Venera ; Baumbach, Tilo ; Surmeneva, Maria A. ; Surmenev, Roman A. / High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels. In: Journal of Biomedical Materials Research - Part A. 2016.
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