High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

P. Christ, S. Rutzinger, W. Seidel, S. Uchaikin, F. Pröbst, C. Koy, M. O. Glocker

Research output: Contribution to journalArticle

13 Citations (Scopus)


Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus, a very high, mass-independent, detection efficiency is expected from their application in time-of-flight mass spectrometry. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series, using standard sample preparation procedures, shows that the detection limit for insulin (Mr: 5734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF β (Mr: 49,032) and for polyclonal IgG (Mr: ca 150,000) in the high femtomole and low picomole range, respectively, were established.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalEuropean Journal of Mass Spectrometry
Issue number4
Publication statusPublished - 2004



  • Cryogenic detector
  • Detection limit
  • Insulin
  • Time-of-flight mass spectrometry

ASJC Scopus subject areas

  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

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