High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

P. Christ, S. Rutzinger, W. Seidel, S. Uchaikin, F. Pröbst, C. Koy, M. O. Glocker

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus, a very high, mass-independent, detection efficiency is expected from their application in time-of-flight mass spectrometry. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series, using standard sample preparation procedures, shows that the detection limit for insulin (Mr: 5734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF β (Mr: 49,032) and for polyclonal IgG (Mr: ca 150,000) in the high femtomole and low picomole range, respectively, were established.

Original languageEnglish
Pages (from-to)469-476
Number of pages8
JournalEuropean Journal of Mass Spectrometry
Volume10
Issue number4
DOIs
Publication statusPublished - 2004

Fingerprint

Cryogenics
Ionization
cryogenics
Mass spectrometry
Desorption
mass spectroscopy
desorption
Detectors
ionization
Lasers
Thermometers
detectors
Mass spectrometers
matrices
Dilution
lasers
insulin
Immunoglobulin G
Phase transitions
thermometers

Keywords

  • Cryogenic detector
  • Detection limit
  • Insulin
  • MALDI
  • Time-of-flight mass spectrometry

ASJC Scopus subject areas

  • Spectroscopy
  • Atomic and Molecular Physics, and Optics

Cite this

High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. / Christ, P.; Rutzinger, S.; Seidel, W.; Uchaikin, S.; Pröbst, F.; Koy, C.; Glocker, M. O.

In: European Journal of Mass Spectrometry, Vol. 10, No. 4, 2004, p. 469-476.

Research output: Contribution to journalArticle

@article{117549e728fb46a2b6cbabc345a5355b,
title = "High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry",
abstract = "Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus, a very high, mass-independent, detection efficiency is expected from their application in time-of-flight mass spectrometry. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series, using standard sample preparation procedures, shows that the detection limit for insulin (Mr: 5734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF β (Mr: 49,032) and for polyclonal IgG (Mr: ca 150,000) in the high femtomole and low picomole range, respectively, were established.",
keywords = "Cryogenic detector, Detection limit, Insulin, MALDI, Time-of-flight mass spectrometry",
author = "P. Christ and S. Rutzinger and W. Seidel and S. Uchaikin and F. Pr{\"o}bst and C. Koy and Glocker, {M. O.}",
year = "2004",
doi = "10.1255/ejms.654",
language = "English",
volume = "10",
pages = "469--476",
journal = "European Journal of Mass Spectrometry",
issn = "1469-0667",
publisher = "I M Publications",
number = "4",

}

TY - JOUR

T1 - High detection sensitivity achieved with cryogenic detectors in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

AU - Christ, P.

AU - Rutzinger, S.

AU - Seidel, W.

AU - Uchaikin, S.

AU - Pröbst, F.

AU - Koy, C.

AU - Glocker, M. O.

PY - 2004

Y1 - 2004

N2 - Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus, a very high, mass-independent, detection efficiency is expected from their application in time-of-flight mass spectrometry. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series, using standard sample preparation procedures, shows that the detection limit for insulin (Mr: 5734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF β (Mr: 49,032) and for polyclonal IgG (Mr: ca 150,000) in the high femtomole and low picomole range, respectively, were established.

AB - Cryogenic detectors directly measure the impact energy of any impinging particle independent of its velocity. Thus, a very high, mass-independent, detection efficiency is expected from their application in time-of-flight mass spectrometry. The cryogenic detector applied here is based on a superconducting phase-transition thermometer and was implemented in a dual reflector time-of-flight mass spectrometer (N-geometry). A dilution series, using standard sample preparation procedures, shows that the detection limit for insulin (Mr: 5734) can be decreased by several orders of magnitude, down to 0.5 amol on the MALDI target. Detection limits for rhM-CSF β (Mr: 49,032) and for polyclonal IgG (Mr: ca 150,000) in the high femtomole and low picomole range, respectively, were established.

KW - Cryogenic detector

KW - Detection limit

KW - Insulin

KW - MALDI

KW - Time-of-flight mass spectrometry

UR - http://www.scopus.com/inward/record.url?scp=4644343974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644343974&partnerID=8YFLogxK

U2 - 10.1255/ejms.654

DO - 10.1255/ejms.654

M3 - Article

VL - 10

SP - 469

EP - 476

JO - European Journal of Mass Spectrometry

JF - European Journal of Mass Spectrometry

SN - 1469-0667

IS - 4

ER -