Abstract
Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.
| Original language | English |
|---|---|
| Pages (from-to) | 187-193 |
| Number of pages | 7 |
| Journal | Biologicheskie Membrany |
| Volume | 33 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2016 |
| Externally published | Yes |
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Keywords
- DARpin9-29
- Fluorescence
- HER2
- PEGylated DARpin-liposomes
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
Cite this
HER2∼specific DARPin-Liposomes : Fabrication and characterization. / Guryev, E. L.; Antonova, N. O.; Yudintsev, A. V.; Tretyakov, A. A.; Stremovskiy, O. A.; Shulga, A. A.; Balalaeva, I. V.; Deyev, S. M.; Zvyagin, A. V.
In: Biologicheskie Membrany, Vol. 33, No. 3, 2016, p. 187-193.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HER2∼specific DARPin-Liposomes
T2 - Fabrication and characterization
AU - Guryev, E. L.
AU - Antonova, N. O.
AU - Yudintsev, A. V.
AU - Tretyakov, A. A.
AU - Stremovskiy, O. A.
AU - Shulga, A. A.
AU - Balalaeva, I. V.
AU - Deyev, S. M.
AU - Zvyagin, A. V.
PY - 2016
Y1 - 2016
N2 - Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.
AB - Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.
KW - DARpin9-29
KW - Fluorescence
KW - HER2
KW - PEGylated DARpin-liposomes
UR - http://www.scopus.com/inward/record.url?scp=84984677258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84984677258&partnerID=8YFLogxK
U2 - 10.7868/S023347551603004X
DO - 10.7868/S023347551603004X
M3 - Article
VL - 33
SP - 187
EP - 193
JO - Biologicheskie Membrany
JF - Biologicheskie Membrany
SN - 0233-4755
IS - 3
ER -