HER2∼specific DARPin-Liposomes: Fabrication and characterization

Abstract

Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.

Original language	English
Pages (from-to)	187-193
Number of pages	7
Journal	Biologicheskie Membrany
Volume	33
Issue number	3
DOIs	https://doi.org/10.7868/S023347551603004X
Publication status	Published - 2016
Externally published	Yes

Keywords

DARpin9-29
Fluorescence
HER2
PEGylated DARpin-liposomes

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.7868/S023347551603004X

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Guryev, E. L., Antonova, N. O., Yudintsev, A. V., Tretyakov, A. A., Stremovskiy, O. A., Shulga, A. A., Balalaeva, I. V., Deyev, S. M., & Zvyagin, A. V. (2016). HER2∼specific DARPin-Liposomes: Fabrication and characterization. Biologicheskie Membrany, 33(3), 187-193. https://doi.org/10.7868/S023347551603004X

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title = "HER2∼specific DARPin-Liposomes: Fabrication and characterization",

abstract = "Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.",

keywords = "DARpin9-29, Fluorescence, HER2, PEGylated DARpin-liposomes",

author = "Guryev, {E. L.} and Antonova, {N. O.} and Yudintsev, {A. V.} and Tretyakov, {A. A.} and Stremovskiy, {O. A.} and Shulga, {A. A.} and Balalaeva, {I. V.} and Deyev, {S. M.} and Zvyagin, {A. V.}",

year = "2016",

doi = "10.7868/S023347551603004X",

language = "English",

volume = "33",

pages = "187--193",

journal = "Biologicheskie Membrany",

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T2 - Fabrication and characterization

AU - Guryev, E. L.

AU - Antonova, N. O.

AU - Yudintsev, A. V.

AU - Tretyakov, A. A.

AU - Stremovskiy, O. A.

AU - Shulga, A. A.

AU - Balalaeva, I. V.

AU - Deyev, S. M.

AU - Zvyagin, A. V.

PY - 2016

Y1 - 2016

N2 - Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.

AB - Liposomes based on natural lipids and their analogues are regarded as promising agents for targeted delivery of antitumor drugs. We have created and characterized in vitro DARPin-liposomes specifically interacting with HER2 cancer marker expressed on the surface of many types of cancer cells. The surface of the liposomes was coated with polyethylene glycol to increase the circulation time of the liposomes in the bloodstream and to reduce nonspecific uptake by macrophages. As a targeting agent we used high- Affinity nonim- munoglobulin peptide DARPin9-29, specific to HER2 receptor. We anticipate that the created DARPin-li- posomes can be used for targeted delivery of anticancer drugs with different mechanisms of action. Besides, the incorporation of fluorescent label rhodamine covalently bound to phospholipids offers the possibility of applying the DARPin-liposomes for diagnostic purposes. We suggest that DARPin-liposomes represent a perspective system for further development and creation of multifunctional liposomal formulations.

KW - DARpin9-29

KW - Fluorescence

KW - HER2

KW - PEGylated DARpin-liposomes

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