Group-selective immunoassay

S. A. Yazynin, S. M. Deev

Research output: Contribution to journalArticlepeer-review

Abstract

The new solid-phase immunoassay technique has been applied for antidigoxin monoclonal antibodies (Mabs) detection. At the first assay step, antidigoxin Mabs (IgG1, K(aff) = 9.2·109 M-1) bound with specially prepared immunosorbent: microtiter plates with adsorbed digoxin-human serum albumin conjugate, in which free amino groups were blocked by glutaraldehyde (the microtiter plates with immobilized conjugate were treated for 3 h by 2.5% glutaraldehyde at 45°C, this had no significant effect on the immunological properties of immunosorbents). After antigen-antibody reaction and solid phase separation, free amino groups were located only at the antidigoxin Mabs bound to GSI immunosorbent. At the second assay step, N-hydroxysuccinimidebiotin ester solution was added to bind with the free amino groups. After separation at the third assay step, biotin labels were detected by the streptavidin-peroxidase conjugate. The method does not require second labeled antibodies and may be used for anti-hapten screening.

Original languageEnglish
Pages (from-to)452-460
Number of pages9
JournalMolekulyarnaya Biologiya
Volume29
Issue number2
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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