Abstract
Aim. The aim of this pilot study was to acquire insight into the parameters of glycaemic control, especially, (1) the time delay (lag phase) between plasma and tissue glucose concentrations in relation to rise and fall in glucose levels and (2) the rate of glucose increase and decrease. Methods. Four healthy people (HP), 4 people with type 1diabetes (DM1) and 4 with type 2 diabetes (DM2) underwent concurrent glucose measurements by means of (1) the continuous glucose monitoring system (CGMS-Medtronic), Medtronic-Minimed, CA, USA, calibrated by the glucometer Calla, Wellion, Austria, and, (2) the Beckman II analyser to measure glucose concentrations in venous plasma. Samples were taken on 4 consecutive days in the fasting state and 4 times after consumption of 50 g glucose. Carelink Personal, MS Excel, Maple and Mat lab were applied to plot the evolution of glucose concentration and analyse the results. The time difference between increase and decrease was calculated for HP, DM 1 and DM 2. Results. In DM1and DM2, glucose tolerance testing (GTT) resulted in slower transport of glucose into subcutaneous tissue than in HP where the lag phase lasted up to 12 min. The maximum increase/decrease rates in DM1 and DM2 vs HP were 0.25 vs < 0.1 mmol/L/min. Conclusion. CGMS is shown to provide reliable plasma glucose concentrations provided the system is calibrated during a steady state. The analysis of glucose change rates improves understanding of metabolic processes better than standard GTT.
| Original language | English |
|---|---|
| Pages (from-to) | 527-534 |
| Number of pages | 8 |
| Journal | Biomedical Papers |
| Volume | 159 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Jan 2015 |
| Externally published | Yes |
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Keywords
- Calibration
- Continuous glucose monitoring
- Diabetes mellitus
- Glucose transport
- Lag phase
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Glucose concentrations in blood and tissue – A pilot study on variable time lag. / Chlup, Rudolf; Krejci, Jan; O’Connell, Mark; Sebestova, Blanka; Plicka, Robert; Jezova, Lucie; Brozova, Tereza; Doubravova, Blanka; Zalesakova, Hana; Durajkova, Emilia; Vojtek, Jiri; Bartek, Josef.
In: Biomedical Papers, Vol. 159, No. 4, 01.01.2015, p. 527-534.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Glucose concentrations in blood and tissue – A pilot study on variable time lag
AU - Chlup, Rudolf
AU - Krejci, Jan
AU - O’Connell, Mark
AU - Sebestova, Blanka
AU - Plicka, Robert
AU - Jezova, Lucie
AU - Brozova, Tereza
AU - Doubravova, Blanka
AU - Zalesakova, Hana
AU - Durajkova, Emilia
AU - Vojtek, Jiri
AU - Bartek, Josef
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Aim. The aim of this pilot study was to acquire insight into the parameters of glycaemic control, especially, (1) the time delay (lag phase) between plasma and tissue glucose concentrations in relation to rise and fall in glucose levels and (2) the rate of glucose increase and decrease. Methods. Four healthy people (HP), 4 people with type 1diabetes (DM1) and 4 with type 2 diabetes (DM2) underwent concurrent glucose measurements by means of (1) the continuous glucose monitoring system (CGMS-Medtronic), Medtronic-Minimed, CA, USA, calibrated by the glucometer Calla, Wellion, Austria, and, (2) the Beckman II analyser to measure glucose concentrations in venous plasma. Samples were taken on 4 consecutive days in the fasting state and 4 times after consumption of 50 g glucose. Carelink Personal, MS Excel, Maple and Mat lab were applied to plot the evolution of glucose concentration and analyse the results. The time difference between increase and decrease was calculated for HP, DM 1 and DM 2. Results. In DM1and DM2, glucose tolerance testing (GTT) resulted in slower transport of glucose into subcutaneous tissue than in HP where the lag phase lasted up to 12 min. The maximum increase/decrease rates in DM1 and DM2 vs HP were 0.25 vs < 0.1 mmol/L/min. Conclusion. CGMS is shown to provide reliable plasma glucose concentrations provided the system is calibrated during a steady state. The analysis of glucose change rates improves understanding of metabolic processes better than standard GTT.
AB - Aim. The aim of this pilot study was to acquire insight into the parameters of glycaemic control, especially, (1) the time delay (lag phase) between plasma and tissue glucose concentrations in relation to rise and fall in glucose levels and (2) the rate of glucose increase and decrease. Methods. Four healthy people (HP), 4 people with type 1diabetes (DM1) and 4 with type 2 diabetes (DM2) underwent concurrent glucose measurements by means of (1) the continuous glucose monitoring system (CGMS-Medtronic), Medtronic-Minimed, CA, USA, calibrated by the glucometer Calla, Wellion, Austria, and, (2) the Beckman II analyser to measure glucose concentrations in venous plasma. Samples were taken on 4 consecutive days in the fasting state and 4 times after consumption of 50 g glucose. Carelink Personal, MS Excel, Maple and Mat lab were applied to plot the evolution of glucose concentration and analyse the results. The time difference between increase and decrease was calculated for HP, DM 1 and DM 2. Results. In DM1and DM2, glucose tolerance testing (GTT) resulted in slower transport of glucose into subcutaneous tissue than in HP where the lag phase lasted up to 12 min. The maximum increase/decrease rates in DM1 and DM2 vs HP were 0.25 vs < 0.1 mmol/L/min. Conclusion. CGMS is shown to provide reliable plasma glucose concentrations provided the system is calibrated during a steady state. The analysis of glucose change rates improves understanding of metabolic processes better than standard GTT.
KW - Calibration
KW - Continuous glucose monitoring
KW - Diabetes mellitus
KW - Glucose transport
KW - Lag phase
UR - http://www.scopus.com/inward/record.url?scp=84949682670&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84949682670&partnerID=8YFLogxK
U2 - 10.5507/bp.2015.007
DO - 10.5507/bp.2015.007
M3 - Article
VL - 159
SP - 527
EP - 534
JO - Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
JF - Biomedical papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
SN - 1213-8118
IS - 4
ER -