[Genetically encoded photoimmunosensitizer].

E. O. Serebrovskaia, O. A. Stremovskiǐ, D. M. Chudakov, K. A. Luk'ianov, S. M. Deev

Research output: Contribution to journalArticlepeer-review

Abstract

Photosensitizer-antibody conjugates are successfully used for targeted elimination of cancer cells bearing specific membrane markers. This method is known as photoimmunotherapy. However, chemical conjugation of photosensitizer and antibody poses a number of complications such as low reproducibility, aggregation and unconjugated photosensitizer impurities. Here we report a fully genetically encoded photoimmunosensitizer, consisting of an anti-HER2/neu miniantibody 4D5scFv and a phototoxic fluorescent protein KillerRed. Both domains in this photoimmunosensitizer retained their functional qualities - high affinity for HER2/neu antigen and phototoxicity respectively. 4D5scFv-KillerRed fusion protein showed high specificity for HER2/neu-over-expressing cells and effectively lowered their viability upon illumination.

Original languageEnglish
Pages (from-to)137-144
Number of pages8
JournalBioorganicheskaia khimiia
Volume37
Issue number1
Publication statusPublished - Jan 2011

ASJC Scopus subject areas

  • Medicine(all)

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