Genetically encoded immunophotosensitizer

E. O. Serebrovskaya, O. A. Stremovsky, D. M. Chudakov, K. A. Lukyanov, S. M. Deyev

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Conjugates of antibodies with photosensitizers are successfully used for the targeted killing of cancer cells bearing particular surface markers by the method known as photoimmunotherapy. However, the chemical conjugation of photosensitizers with antibodies poses a number of problems. Among these are a low reproducibility, aggregation, and the presence of impurities of the unconjugated photosensitizer. Here we describe a method of designing a fully genetically encoded immunophotosensitizer, which consists of the anti-HER2/neu miniantibody 4D5scFv as a targeting molecule and the phototoxic fluorescent protein KillerRed as a photosensitizing molecule. Both domains in the recombinant protein retained their functional properties: a high affinity for the HER2/neu antigen and phototoxicity. The recombinant protein 4D5scFv-KillerRed showed a high specificity for HER2/neu-overexpressing cancer cells and effectively lowered their viability upon irradiation.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalRussian Journal of Bioorganic Chemistry
Volume37
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Photosensitizing Agents
Recombinant Proteins
Bearings (structural)
Cells
Phototoxic Dermatitis
Molecules
Antibodies
Neoplasms
Agglomeration
Irradiation
Impurities
Antigens
Proteins

Keywords

  • fluorescent protein
  • HER2/neu
  • miniantibody
  • photoimmunotherapy
  • photosensitizer

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

Cite this

Serebrovskaya, E. O., Stremovsky, O. A., Chudakov, D. M., Lukyanov, K. A., & Deyev, S. M. (2011). Genetically encoded immunophotosensitizer. Russian Journal of Bioorganic Chemistry, 37(1), 123-129. https://doi.org/10.1134/S1068162011010134

Genetically encoded immunophotosensitizer. / Serebrovskaya, E. O.; Stremovsky, O. A.; Chudakov, D. M.; Lukyanov, K. A.; Deyev, S. M.

In: Russian Journal of Bioorganic Chemistry, Vol. 37, No. 1, 01.2011, p. 123-129.

Research output: Contribution to journalArticle

Serebrovskaya, EO, Stremovsky, OA, Chudakov, DM, Lukyanov, KA & Deyev, SM 2011, 'Genetically encoded immunophotosensitizer', Russian Journal of Bioorganic Chemistry, vol. 37, no. 1, pp. 123-129. https://doi.org/10.1134/S1068162011010134
Serebrovskaya, E. O. ; Stremovsky, O. A. ; Chudakov, D. M. ; Lukyanov, K. A. ; Deyev, S. M. / Genetically encoded immunophotosensitizer. In: Russian Journal of Bioorganic Chemistry. 2011 ; Vol. 37, No. 1. pp. 123-129.
@article{e361bdb783dc4ba6878edd8637b7e807,
title = "Genetically encoded immunophotosensitizer",
abstract = "Conjugates of antibodies with photosensitizers are successfully used for the targeted killing of cancer cells bearing particular surface markers by the method known as photoimmunotherapy. However, the chemical conjugation of photosensitizers with antibodies poses a number of problems. Among these are a low reproducibility, aggregation, and the presence of impurities of the unconjugated photosensitizer. Here we describe a method of designing a fully genetically encoded immunophotosensitizer, which consists of the anti-HER2/neu miniantibody 4D5scFv as a targeting molecule and the phototoxic fluorescent protein KillerRed as a photosensitizing molecule. Both domains in the recombinant protein retained their functional properties: a high affinity for the HER2/neu antigen and phototoxicity. The recombinant protein 4D5scFv-KillerRed showed a high specificity for HER2/neu-overexpressing cancer cells and effectively lowered their viability upon irradiation.",
keywords = "fluorescent protein, HER2/neu, miniantibody, photoimmunotherapy, photosensitizer",
author = "Serebrovskaya, {E. O.} and Stremovsky, {O. A.} and Chudakov, {D. M.} and Lukyanov, {K. A.} and Deyev, {S. M.}",
year = "2011",
month = "1",
doi = "10.1134/S1068162011010134",
language = "English",
volume = "37",
pages = "123--129",
journal = "Russian Journal of Bioorganic Chemistry",
issn = "1068-1620",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "1",

}

TY - JOUR

T1 - Genetically encoded immunophotosensitizer

AU - Serebrovskaya, E. O.

AU - Stremovsky, O. A.

AU - Chudakov, D. M.

AU - Lukyanov, K. A.

AU - Deyev, S. M.

PY - 2011/1

Y1 - 2011/1

N2 - Conjugates of antibodies with photosensitizers are successfully used for the targeted killing of cancer cells bearing particular surface markers by the method known as photoimmunotherapy. However, the chemical conjugation of photosensitizers with antibodies poses a number of problems. Among these are a low reproducibility, aggregation, and the presence of impurities of the unconjugated photosensitizer. Here we describe a method of designing a fully genetically encoded immunophotosensitizer, which consists of the anti-HER2/neu miniantibody 4D5scFv as a targeting molecule and the phototoxic fluorescent protein KillerRed as a photosensitizing molecule. Both domains in the recombinant protein retained their functional properties: a high affinity for the HER2/neu antigen and phototoxicity. The recombinant protein 4D5scFv-KillerRed showed a high specificity for HER2/neu-overexpressing cancer cells and effectively lowered their viability upon irradiation.

AB - Conjugates of antibodies with photosensitizers are successfully used for the targeted killing of cancer cells bearing particular surface markers by the method known as photoimmunotherapy. However, the chemical conjugation of photosensitizers with antibodies poses a number of problems. Among these are a low reproducibility, aggregation, and the presence of impurities of the unconjugated photosensitizer. Here we describe a method of designing a fully genetically encoded immunophotosensitizer, which consists of the anti-HER2/neu miniantibody 4D5scFv as a targeting molecule and the phototoxic fluorescent protein KillerRed as a photosensitizing molecule. Both domains in the recombinant protein retained their functional properties: a high affinity for the HER2/neu antigen and phototoxicity. The recombinant protein 4D5scFv-KillerRed showed a high specificity for HER2/neu-overexpressing cancer cells and effectively lowered their viability upon irradiation.

KW - fluorescent protein

KW - HER2/neu

KW - miniantibody

KW - photoimmunotherapy

KW - photosensitizer

UR - http://www.scopus.com/inward/record.url?scp=79952654669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952654669&partnerID=8YFLogxK

U2 - 10.1134/S1068162011010134

DO - 10.1134/S1068162011010134

M3 - Article

VL - 37

SP - 123

EP - 129

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 1

ER -