Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia

Yu B. Lishmanov, N. V. Naryzhnaya, L. N. Maslov, E. S. Prokudina, A. S. Gorbunov, S. Yu Tsibulnikov

Research Nuclear Reactor Center

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Abstract

Parameters of respiration, transmembrane potential, and Ca ²⁺-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca ²⁺-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.

Original language	English
Pages (from-to)	645-648
Number of pages	4
Journal	Bulletin of Experimental Biology and Medicine
Volume	156
Issue number	5
DOIs	https://doi.org/10.1007/s10517-014-2416-1
Publication status	Published - 1 Jan 2014

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Keywords

adaptation to hypoxia
heart
ischemia
mitochondria

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lishmanov, Y. B., Naryzhnaya, N. V., Maslov, L. N., Prokudina, E. S., Gorbunov, A. S., & Tsibulnikov, S. Y. (2014). Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia. Bulletin of Experimental Biology and Medicine, 156(5), 645-648. https://doi.org/10.1007/s10517-014-2416-1

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abstract = "Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.",

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AU - Lishmanov, Yu B.

AU - Naryzhnaya, N. V.

AU - Maslov, L. N.

AU - Prokudina, E. S.

AU - Gorbunov, A. S.

AU - Tsibulnikov, S. Yu

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AB - Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.

KW - adaptation to hypoxia

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10.1007/s10517-014-2416-1