Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia

Abstract

Parameters of respiration, transmembrane potential, and Ca ²⁺-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca ²⁺-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.

Original language	English
Pages (from-to)	645-648
Number of pages	4
Journal	Bulletin of Experimental Biology and Medicine
Volume	156
Issue number	5
DOIs	https://doi.org/10.1007/s10517-014-2416-1
Publication status	Published - 1 Jan 2014

Keywords

adaptation to hypoxia
heart
ischemia
mitochondria

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1007/s10517-014-2416-1

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Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia. / Lishmanov, Yu B.; Naryzhnaya, N. V.; Maslov, L. N.; Prokudina, E. S.; Gorbunov, A. S.; Tsibulnikov, S. Yu.

In: Bulletin of Experimental Biology and Medicine, Vol. 156, No. 5, 01.01.2014, p. 645-648.

Research output: Contribution to journal › Article › peer-review

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title = "Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia",

abstract = "Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.",

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AU - Lishmanov, Yu B.

AU - Naryzhnaya, N. V.

AU - Maslov, L. N.

AU - Prokudina, E. S.

AU - Gorbunov, A. S.

AU - Tsibulnikov, S. Yu

PY - 2014/1/1

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N2 - Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.

AB - Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.

KW - adaptation to hypoxia

KW - heart

KW - ischemia

KW - mitochondria

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