Abstract
Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.
| Original language | English |
|---|---|
| Pages (from-to) | 645-648 |
| Number of pages | 4 |
| Journal | Bulletin of Experimental Biology and Medicine |
| Volume | 156 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 Jan 2014 |
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Keywords
- adaptation to hypoxia
- heart
- ischemia
- mitochondria
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia. / Lishmanov, Yu B.; Naryzhnaya, N. V.; Maslov, L. N.; Prokudina, E. S.; Gorbunov, A. S.; Tsibulnikov, S. Yu.
In: Bulletin of Experimental Biology and Medicine, Vol. 156, No. 5, 01.01.2014, p. 645-648.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Functional state of myocardial mitochondria in ischemia reperfusion of the heart in rats adapted to hypoxia
AU - Lishmanov, Yu B.
AU - Naryzhnaya, N. V.
AU - Maslov, L. N.
AU - Prokudina, E. S.
AU - Gorbunov, A. S.
AU - Tsibulnikov, S. Yu
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.
AB - Parameters of respiration, transmembrane potential, and Ca 2+-binding capacity of mitochondria isolated from Langendorff-perfused hearts of rats adapted to normobaric hypoxia were analyzed. Ischemia and reperfusion modeling in intact and adapted animals reduced Ca 2+-binding capacity of mitochondria, which indicated increased sensitivity of mitochondrial permeability transition pores (MPTP) to calcium ions. These changes were accompanied by a decrease in transmembrane potential, ADP/O coeffi cient (ratio of added ADP to oxygen consumption in State 3), and inhibition of State 3 respiration. At the same time, adaptation attenuated the negative effect of ischemia and reperfusion on the functional state of mitochondria.
KW - adaptation to hypoxia
KW - heart
KW - ischemia
KW - mitochondria
UR - http://www.scopus.com/inward/record.url?scp=84898823712&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898823712&partnerID=8YFLogxK
U2 - 10.1007/s10517-014-2416-1
DO - 10.1007/s10517-014-2416-1
M3 - Article
VL - 156
SP - 645
EP - 648
JO - Bulletin of Experimental Biology and Medicine
JF - Bulletin of Experimental Biology and Medicine
SN - 0007-4888
IS - 5
ER -