Functional, anatomical, and molecular investigation of the cardiac conduction system and arrhythmogenic atrioventricular ring tissue in the rat heart

Andrew J. Atkinson, Sunil J.it R.J. Logantha, Guoliang Hao, Joseph Yanni, Olga Fedorenko, Aditi Sinha, Stephen H. Gilbert, Alan P. Benson, David L. Buckley, Robert H. Anderson, Mark R. Boyett, Halina Dobrzynski

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

BACKGROUND: The cardiac conduction system consists of the sinus node, nodal extensions, atrioventricular (AV) node, penetrating bundle, bundle branches, and Purkinje fibers. Node-like AV ring tissue also exists at the AV junctions, and the right and left rings unite at the retroaortic node. The study aims were to (1) construct a 3-dimensional anatomical model of the AV rings and retroaortic node, (2) map electrical activation in the right ring and study its action potential characteristics, and (3) examine gene expression in the right ring and retroaortic node.

METHODS AND RESULTS: Three-dimensional reconstruction (based on magnetic resonance imaging, histology, and immunohistochemistry) showed the extent and organization of the specialized tissues (eg, how the AV rings form the right and left nodal extensions into the AV node). Multiextracellular electrode array and microelectrode mapping of isolated right ring preparations revealed robust spontaneous activity with characteristic diastolic depolarization. Using laser microdissection gene expression measured at the mRNA level (using quantitative PCR) and protein level (using immunohistochemistry and Western blotting) showed that the right ring and retroaortic node, like the sinus node and AV node but, unlike ventricular muscle, had statistically significant higher expression of key transcription factors (including Tbx3, Msx2, and Id2) and ion channels (including HCN4, Cav3.1, Cav3.2, Kv1.5, SK1, Kir3.1, and Kir3.4) and lower expression of other key ion channels (Nav1.5 and Kir2.1).

CONCLUSIONS: The AV rings and retroaortic node possess gene expression profiles similar to that of the AV node. Ion channel expression and electrophysiological recordings show the AV rings could act as ectopic pacemakers and a source of atrial tachycardia.

Original languageEnglish
Pages (from-to)e000246
JournalJournal of the American Heart Association
Volume2
Issue number6
DOIs
Publication statusPublished - 1 Dec 2013

Fingerprint

Atrioventricular Node
Ion Channels
Sinoatrial Node
Immunohistochemistry
Purkinje Fibers
Anatomic Models
Gene Expression
Microdissection
Microelectrodes
Transcriptome
Tachycardia
Action Potentials
Histology
Electrodes
Lasers
Transcription Factors
Western Blotting
Magnetic Resonance Imaging
Muscles
Polymerase Chain Reaction

Keywords

  • action potential
  • arrhythmogenesis
  • atrioventricular ring tissues
  • cardiac conduction system
  • ion channels

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Functional, anatomical, and molecular investigation of the cardiac conduction system and arrhythmogenic atrioventricular ring tissue in the rat heart. / Atkinson, Andrew J.; Logantha, Sunil J.it R.J.; Hao, Guoliang; Yanni, Joseph; Fedorenko, Olga; Sinha, Aditi; Gilbert, Stephen H.; Benson, Alan P.; Buckley, David L.; Anderson, Robert H.; Boyett, Mark R.; Dobrzynski, Halina.

In: Journal of the American Heart Association, Vol. 2, No. 6, 01.12.2013, p. e000246.

Research output: Contribution to journalArticle

Atkinson, AJ, Logantha, SJIRJ, Hao, G, Yanni, J, Fedorenko, O, Sinha, A, Gilbert, SH, Benson, AP, Buckley, DL, Anderson, RH, Boyett, MR & Dobrzynski, H 2013, 'Functional, anatomical, and molecular investigation of the cardiac conduction system and arrhythmogenic atrioventricular ring tissue in the rat heart', Journal of the American Heart Association, vol. 2, no. 6, pp. e000246. https://doi.org/10.1161/JAHA.113.000246
Atkinson, Andrew J. ; Logantha, Sunil J.it R.J. ; Hao, Guoliang ; Yanni, Joseph ; Fedorenko, Olga ; Sinha, Aditi ; Gilbert, Stephen H. ; Benson, Alan P. ; Buckley, David L. ; Anderson, Robert H. ; Boyett, Mark R. ; Dobrzynski, Halina. / Functional, anatomical, and molecular investigation of the cardiac conduction system and arrhythmogenic atrioventricular ring tissue in the rat heart. In: Journal of the American Heart Association. 2013 ; Vol. 2, No. 6. pp. e000246.
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AU - Hao, Guoliang

AU - Yanni, Joseph

AU - Fedorenko, Olga

AU - Sinha, Aditi

AU - Gilbert, Stephen H.

AU - Benson, Alan P.

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