Force spectroscopy of barnase-barstar single molecule interaction

S. K. Sekatskii, M. Favre, G. Dietler, A. G. Mikhailov, D. V. Klinov, S. V. Lukash, S. M. Deyev

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Results of the single molecule force spectroscopy study of specific interactions between ribonuclease barnase and its inhibitor barstar are presented. Experimental data obtained for the force loading rate ranging 2-70 nN/s are well approximated by a single straight line, from which the dissociation barrier of the width of 0.12 nm and height of 0.75-0.85×10-19 J can be inferred. The measured value of specific interaction does not depend on the NaCl concentration. This apparently contradicts the well-known dependence of the binding energy of this pair on the salt concentration, but such a "contradiction" is explained by the insensitivity of the force spectroscopy data to the relatively long-range electrostatic interaction. The latter essentially contributes to the value of barnase-barstar binding energy revealed by biochemical measurements, and it is exactly this electrostatic interaction which is influenced by the salt concentration.

Original languageEnglish
Pages (from-to)583-588
Number of pages6
JournalJournal of Molecular Recognition
Volume23
Issue number6
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Fingerprint

Static Electricity
Spectrum Analysis
Salts
Ribonucleases
Bacillus amyloliquefaciens barstar protein
Bacillus amyloliquefaciens ribonuclease
Single Molecule Imaging

Keywords

  • Barnase
  • Barstar
  • Force spectroscopy
  • Ligand-receptor interactions

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Sekatskii, S. K., Favre, M., Dietler, G., Mikhailov, A. G., Klinov, D. V., Lukash, S. V., & Deyev, S. M. (2010). Force spectroscopy of barnase-barstar single molecule interaction. Journal of Molecular Recognition, 23(6), 583-588. https://doi.org/10.1002/jmr.1030

Force spectroscopy of barnase-barstar single molecule interaction. / Sekatskii, S. K.; Favre, M.; Dietler, G.; Mikhailov, A. G.; Klinov, D. V.; Lukash, S. V.; Deyev, S. M.

In: Journal of Molecular Recognition, Vol. 23, No. 6, 11.2010, p. 583-588.

Research output: Contribution to journalArticle

Sekatskii, SK, Favre, M, Dietler, G, Mikhailov, AG, Klinov, DV, Lukash, SV & Deyev, SM 2010, 'Force spectroscopy of barnase-barstar single molecule interaction', Journal of Molecular Recognition, vol. 23, no. 6, pp. 583-588. https://doi.org/10.1002/jmr.1030
Sekatskii SK, Favre M, Dietler G, Mikhailov AG, Klinov DV, Lukash SV et al. Force spectroscopy of barnase-barstar single molecule interaction. Journal of Molecular Recognition. 2010 Nov;23(6):583-588. https://doi.org/10.1002/jmr.1030
Sekatskii, S. K. ; Favre, M. ; Dietler, G. ; Mikhailov, A. G. ; Klinov, D. V. ; Lukash, S. V. ; Deyev, S. M. / Force spectroscopy of barnase-barstar single molecule interaction. In: Journal of Molecular Recognition. 2010 ; Vol. 23, No. 6. pp. 583-588.
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