Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment

Tatiana A. Zdobnova, Sergey G. Dorofeev, Piter N. Tananaev, Roman B. Vasiliev, Taras G. Balandin, Eveline F. Edelweiss, Oleg A. Stremovskiy, Irina V. Balalaeva, Ilya V. Turchin, Ekaterina N. Lebedenko, Vladimir P. Zlomanov, Sergey M. Deyev

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28 Citations (Scopus)

Abstract

Semiconductor quantum dots (QDs) coupled with cancer-specific targeting ligands are new promising agents for fluorescent visualization of cancer cells. Human epidermal growth factor receptor 2/neu (HER2/neu), overexpressed on the surface of many cancer cells, is an important target for cancer diagnostics. Antibody scFv fragments as a targeting agent for direct delivery of fluorophores offer significant advantages over full-size antibodies due to their small size, lower cross-reactivity, and immunogenicity. We have used quantum dots linked to anti-HER2/neu 4D5 scFv antibody to label HER2/neu-overexpressing live cells. Labeling of target cells was shown to have high brightness, photostability, and specificity. The results indicate that construction based on quantum dots and scFv antibody can be successfully used for cancer cell visualization.

Original languageEnglish
Article number021004
JournalJournal of Biomedical Optics
Volume14
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

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Keywords

  • 4D5 scFv antibody
  • human breast cancer SKBR-3 cells
  • human epidermal growth factor receptor 2/neu (HER2/neu)
  • immunolabeling
  • quantum dots (QDs)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Zdobnova, T. A., Dorofeev, S. G., Tananaev, P. N., Vasiliev, R. B., Balandin, T. G., Edelweiss, E. F., ... Deyev, S. M. (2009). Fluorescent immunolabeling of cancer cells by quantum dots and antibody scFv fragment. Journal of Biomedical Optics, 14(2), [021004]. https://doi.org/10.1117/1.3122775