Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer

E. I. Shramova, G. M. Proshkina, S. P. Chumakov, Yu M. Khodarovich, S. M. Deyev

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.

Original languageEnglish
Pages (from-to)118-125
Number of pages8
JournalActa Naturae
Volume8
Issue number4
Publication statusPublished - 2016

Keywords

  • Bioluminescence resonance energy transfer
  • BRET - bioluminescence resonance energy transfer
  • Flavoprotein miniSOG
  • FMN - flavin mononucleotide
  • NanoLuc luciferase
  • PCR - polymerase chain reaction
  • PDT - photodynamic therapy
  • Photodynamic therapy
  • ROS - reactive oxygen species

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

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  • Cite this

    Shramova, E. I., Proshkina, G. M., Chumakov, S. P., Khodarovich, Y. M., & Deyev, S. M. (2016). Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer. Acta Naturae, 8(4), 118-125.