Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization

E. I. Shramova, G. M. Proshkina, S. M. Deyev, R. V. Petrov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.

Original languageEnglish
Pages (from-to)228-230
Number of pages3
JournalDoklady Biochemistry and Biophysics
Volume474
Issue number1
DOIs
Publication statusPublished - 1 May 2017
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Chemistry(all)
  • Medicine(all)
  • Biochemistry

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