Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization

E. I. Shramova, G. M. Proshkina, S. M. Deyev, R. V. Petrov

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.

Original languageEnglish
Pages (from-to)228-230
Number of pages3
JournalDoklady Biochemistry and Biophysics
Volume474
Issue number1
DOIs
Publication statusPublished - 1 May 2017
Externally publishedYes

Fingerprint

Membrane Fusion Proteins
Flavoproteins
Mitochondrial Proteins
Energy Transfer
Photochemotherapy
Cytotoxicity
Luciferases
Light
Membranes
Fusion reactions
Neoplasms
Photodynamic therapy
Proteins
Energy transfer
Light sources
Substrates
In Vitro Techniques

ASJC Scopus subject areas

  • Biophysics
  • Chemistry(all)
  • Medicine(all)
  • Biochemistry

Cite this

Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization. / Shramova, E. I.; Proshkina, G. M.; Deyev, S. M.; Petrov, R. V.

In: Doklady Biochemistry and Biophysics, Vol. 474, No. 1, 01.05.2017, p. 228-230.

Research output: Contribution to journalArticle

Shramova, E. I. ; Proshkina, G. M. ; Deyev, S. M. ; Petrov, R. V. / Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization. In: Doklady Biochemistry and Biophysics. 2017 ; Vol. 474, No. 1. pp. 228-230.
@article{0e3846a943694860bc6507f207c9ac06,
title = "Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization",
abstract = "It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48{\%} for the cytoplasmic localization of the fusion protein, 65{\%} for the mitochondrial localization, and 69{\%} for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.",
author = "Shramova, {E. I.} and Proshkina, {G. M.} and Deyev, {S. M.} and Petrov, {R. V.}",
year = "2017",
month = "5",
day = "1",
doi = "10.1134/S160767291703019X",
language = "English",
volume = "474",
pages = "228--230",
journal = "Doklady Biochemistry and Biophysics",
issn = "1607-6729",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "1",

}

TY - JOUR

T1 - Flavoprotein miniSOG BRET-induced cytotoxicity depends on its intracellular localization

AU - Shramova, E. I.

AU - Proshkina, G. M.

AU - Deyev, S. M.

AU - Petrov, R. V.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.

AB - It is proposed to use the bioluminescent resonance energy transfer to solve the problem of creating the internal light sources in photodynamic therapy of cancer. Energy donor in the developed system is the oxidized form of the luciferase NanoLuc substrate furimamide, and acceptor is the phototoxic fluorescent protein miniSOG. It is shown that, in the proposed system, the photoinduced cytotoxicity of flavoprotein miniSOG in vitro depends on the intracellular localization, and the cytotoxic effect is 48% for the cytoplasmic localization of the fusion protein, 65% for the mitochondrial localization, and 69% for the membrane localization. The obtained data indicate that, for maximization of the photodynamic effect in vivo, it is appropriate to use the NanoLuc–miniSOG fusion protein in the membrane localization.

UR - http://www.scopus.com/inward/record.url?scp=85025064243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025064243&partnerID=8YFLogxK

U2 - 10.1134/S160767291703019X

DO - 10.1134/S160767291703019X

M3 - Article

AN - SCOPUS:85025064243

VL - 474

SP - 228

EP - 230

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

SN - 1607-6729

IS - 1

ER -