Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes

Ekaterina A. Grebenik, Annemarie Nadort, Alla N. Generalova, Andrei V. Nechaev, Varun K.A. Sreenivasan, Evgeny V. Khaydukov, Vladimir A. Semchishen, Alexey P. Popov, Viktor I. Sokolov, Aleksandr S. Akhmanov, Vitali P. Zubov, Dmitry V. Klinov, Vladislav Y. Panchenko, Sergey M. Deyev, Andrei V. Zvyagin

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Innovative luminescent nanomaterials, termed upconversion nanoparticles (UCNPs), have demonstrated considerable promise as molecular probes for high-contrast optical imaging in cells and small animals. The feasibility study of optical diagnostics in humans is reported here based on experimental and theoretical modeling of optical imaging of an UCNP-labeled breast cancer lesion. UCNPs synthesized in-house were surface-capped with an amphiphilic polymer to achieve good colloidal stability in aqueous buffer solutions. The scFv4D5 mini-antibodies were grafted onto the UCNPs via a high-affinity molecular linker barstar:barnase (Bs:Bn) to allow their specific binding to the human epidermal growth factor receptor HER2/neu, which is overexpressed in human breast adenocarcinoma cells SK-BR-3. UCNP-Bs:Bn-scFv4D5 biocomplexes exhibited high-specific immobilization on the SK-BR-3 cells with the optical contrast as high as 10:1 benchmarked against a negative control cell line. Breast cancer optical diagnostics was experimentally modeled by means of epi-luminescence imaging of a monolayer of the UCNP-labeled SK-BR-3 cells buried under a breast tissue mimicking optical phantom. The experimental results were analyzed theoretically and projected to in vivo detection of early-stage breast cancer. The model predicts that the UCNP-assisted cancer detection is feasible up to 4 mm in tissue depth, showing considerable potential for diagnostic and image-guided surgery applications.

Original languageEnglish
Article number076004
JournalJournal of Biomedical Optics
Volume18
Issue number7
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

Fingerprint

breast
lesions
cancer
Nanoparticles
Imaging techniques
nanoparticles
cells
Tissue
Molecular Probes
antibodies
immobilization
cultured cells
Epidermal Growth Factor Receptor
Nanostructured materials
Antibodies
surgery
Surgery
animals
affinity
Luminescence

Keywords

  • biomedical optical imaging
  • breast cancer
  • human epidermal growth factor receptor 2
  • mini-antibody (wild type)
  • tissue phantom
  • upconversion nanoparticle

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this

Grebenik, E. A., Nadort, A., Generalova, A. N., Nechaev, A. V., Sreenivasan, V. K. A., Khaydukov, E. V., ... Zvyagin, A. V. (2013). Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes. Journal of Biomedical Optics, 18(7), [076004]. https://doi.org/10.1117/1.JBO.18.7.076004

Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes. / Grebenik, Ekaterina A.; Nadort, Annemarie; Generalova, Alla N.; Nechaev, Andrei V.; Sreenivasan, Varun K.A.; Khaydukov, Evgeny V.; Semchishen, Vladimir A.; Popov, Alexey P.; Sokolov, Viktor I.; Akhmanov, Aleksandr S.; Zubov, Vitali P.; Klinov, Dmitry V.; Panchenko, Vladislav Y.; Deyev, Sergey M.; Zvyagin, Andrei V.

In: Journal of Biomedical Optics, Vol. 18, No. 7, 076004, 07.2013.

Research output: Contribution to journalArticle

Grebenik, EA, Nadort, A, Generalova, AN, Nechaev, AV, Sreenivasan, VKA, Khaydukov, EV, Semchishen, VA, Popov, AP, Sokolov, VI, Akhmanov, AS, Zubov, VP, Klinov, DV, Panchenko, VY, Deyev, SM & Zvyagin, AV 2013, 'Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes', Journal of Biomedical Optics, vol. 18, no. 7, 076004. https://doi.org/10.1117/1.JBO.18.7.076004
Grebenik, Ekaterina A. ; Nadort, Annemarie ; Generalova, Alla N. ; Nechaev, Andrei V. ; Sreenivasan, Varun K.A. ; Khaydukov, Evgeny V. ; Semchishen, Vladimir A. ; Popov, Alexey P. ; Sokolov, Viktor I. ; Akhmanov, Aleksandr S. ; Zubov, Vitali P. ; Klinov, Dmitry V. ; Panchenko, Vladislav Y. ; Deyev, Sergey M. ; Zvyagin, Andrei V. / Feasibility study of the optical imaging of a breast cancer lesion labeled with upconversion nanoparticle biocomplexes. In: Journal of Biomedical Optics. 2013 ; Vol. 18, No. 7.
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