Fas-induced caspase denitrosylation

Joan B. Mannick, Alfred Hausladen, Limin Liu, Douglas T. Hess, Ming Zeng, Qian X. Miao, Laurie S. Kane, Andrew J. Gow, Jonathan S. Stamler

Research output: Contribution to journalArticle

671 Citations (Scopus)

Abstract

Only a few intracellular S-nitrosylated proteins have been identified, and it is unknown if protein 5-nitrosylation/denitrosylation is a component of signal transduction cascades. Caspase-3 zymogens were found to be S- nitrosylated on their catalytic-site cysteine in unstimulated human cell tines and denitrosylated upon activation of the Fas apoptotic pathway. Decreased caspase-3 S-nitrosylation was associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active-site thiol. Protein S- nitrosylation/denitrosylation can thus serve as a regulatory process in signal transduction pathways.

Original languageEnglish
Pages (from-to)651-654
Number of pages4
JournalScience
Volume284
Issue number5414
DOIs
Publication statusPublished - 23 Apr 1999
Externally publishedYes

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Caspases
Caspase 3
Enzyme Precursors
Protein S
Signal Transduction
Catalytic Domain
Sulfhydryl Compounds
Cysteine
Proteins

ASJC Scopus subject areas

  • General

Cite this

Mannick, J. B., Hausladen, A., Liu, L., Hess, D. T., Zeng, M., Miao, Q. X., ... Stamler, J. S. (1999). Fas-induced caspase denitrosylation. Science, 284(5414), 651-654. https://doi.org/10.1126/science.284.5414.651

Fas-induced caspase denitrosylation. / Mannick, Joan B.; Hausladen, Alfred; Liu, Limin; Hess, Douglas T.; Zeng, Ming; Miao, Qian X.; Kane, Laurie S.; Gow, Andrew J.; Stamler, Jonathan S.

In: Science, Vol. 284, No. 5414, 23.04.1999, p. 651-654.

Research output: Contribution to journalArticle

Mannick, JB, Hausladen, A, Liu, L, Hess, DT, Zeng, M, Miao, QX, Kane, LS, Gow, AJ & Stamler, JS 1999, 'Fas-induced caspase denitrosylation', Science, vol. 284, no. 5414, pp. 651-654. https://doi.org/10.1126/science.284.5414.651
Mannick JB, Hausladen A, Liu L, Hess DT, Zeng M, Miao QX et al. Fas-induced caspase denitrosylation. Science. 1999 Apr 23;284(5414):651-654. https://doi.org/10.1126/science.284.5414.651
Mannick, Joan B. ; Hausladen, Alfred ; Liu, Limin ; Hess, Douglas T. ; Zeng, Ming ; Miao, Qian X. ; Kane, Laurie S. ; Gow, Andrew J. ; Stamler, Jonathan S. / Fas-induced caspase denitrosylation. In: Science. 1999 ; Vol. 284, No. 5414. pp. 651-654.
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AU - Gow, Andrew J.

AU - Stamler, Jonathan S.

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