Facilitated Electron Hopping in Nanolayer Oxygen-Insensitive Glucose Biosensor for Application in a Complex Matrix

Matteo Grattieri, Matteo Tucci, Massimiliano Bestetti, Stefano Trasatti, Pierangela Cristiani

Research output: Contribution to journalArticle

8 Citations (Scopus)


Electrochemical experimental evidence of facilitated electron transfer in a sub-micrometer biosensor is presented. Layer-by-layer self-assembled deposition provides the unique advantage to specifically control the thickness of the biosensors, allowing an oxygen-insensitive device with a film thickness of 70 nanometers to be obtained. The biosensor is based on a poly(allylamine) osmium redox mediator and glucose 1-oxidase. The immobilized enzyme contributes to the signal generation through the full biosensor thickness, with no loss of “active enzyme” in the outer layer of the biosensor through reaction with oxygen, as reported in the case of thick redox hydrogels. The application of the biosensor in complex matrices was approached with tests in wastewater. Encapsulation of the biosensor with Nafion® membrane ensured the protection of the enzyme molecules from the external environment, allowing the successful application of the sensor in a complex matrix.

Original languageEnglish
Pages (from-to)1884-1889
Number of pages6
Issue number11
Publication statusPublished - 1 Nov 2016



  • electron hopping
  • nanostructures
  • oxygen-insensitive biosensors
  • redox osmium polymers
  • wastewater

ASJC Scopus subject areas

  • Catalysis
  • Electrochemistry

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