Expression of single-chain antibody-barstar fusion in plants

Ekaterina G. Semenyuk, Oleg A. Stremovskiy, Evelina F. Edelweiss, Olga V. Shirshikova, Taras G. Balandin, Yaroslav I. Buryanov, Sergey M. Deyev

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We successfully cloned and expressed a single-chain antibody (425scFv), that is directed to human epidermal growth factor receptor HER1 (EGFR) in transgenic tobacco plants as a fusion with bacterial barstar gene (425scFv-barstar). Plant-produced recombinant 425scFv-barstar was recovered using barstar-barnase system. Based on barstar-barnase affinity, during purification of the plant-produced 425scFv-barstar, we generated bispecific scFv-antibody heterodimers from individual single-chain fragments initially produced in different host systems with binding activity to both HER1 and HER2/neu tumor antigens. We demonstrated by flow cytometry and indirect immunofluorescent microscopy that both the components of heterodimer retain its specific cell-binding activity.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalBiochimie
Volume89
Issue number1
DOIs
Publication statusPublished - Jan 2007
Externally publishedYes

Fingerprint

Single-Chain Antibodies
Fusion reactions
Bispecific Antibodies
Bacterial Genes
Tobacco
Flow cytometry
Genetically Modified Plants
Neoplasm Antigens
Epidermal Growth Factor Receptor
Purification
Bacillus amyloliquefaciens barstar protein
Microscopy
Flow Cytometry
Microscopic examination
Genes

Keywords

  • Barnase
  • Barstar
  • HER1 (EGFR)
  • Single-chain Fv antibody
  • Transgenic plants

ASJC Scopus subject areas

  • Biochemistry

Cite this

Semenyuk, E. G., Stremovskiy, O. A., Edelweiss, E. F., Shirshikova, O. V., Balandin, T. G., Buryanov, Y. I., & Deyev, S. M. (2007). Expression of single-chain antibody-barstar fusion in plants. Biochimie, 89(1), 31-38. https://doi.org/10.1016/j.biochi.2006.07.012

Expression of single-chain antibody-barstar fusion in plants. / Semenyuk, Ekaterina G.; Stremovskiy, Oleg A.; Edelweiss, Evelina F.; Shirshikova, Olga V.; Balandin, Taras G.; Buryanov, Yaroslav I.; Deyev, Sergey M.

In: Biochimie, Vol. 89, No. 1, 01.2007, p. 31-38.

Research output: Contribution to journalArticle

Semenyuk, EG, Stremovskiy, OA, Edelweiss, EF, Shirshikova, OV, Balandin, TG, Buryanov, YI & Deyev, SM 2007, 'Expression of single-chain antibody-barstar fusion in plants', Biochimie, vol. 89, no. 1, pp. 31-38. https://doi.org/10.1016/j.biochi.2006.07.012
Semenyuk EG, Stremovskiy OA, Edelweiss EF, Shirshikova OV, Balandin TG, Buryanov YI et al. Expression of single-chain antibody-barstar fusion in plants. Biochimie. 2007 Jan;89(1):31-38. https://doi.org/10.1016/j.biochi.2006.07.012
Semenyuk, Ekaterina G. ; Stremovskiy, Oleg A. ; Edelweiss, Evelina F. ; Shirshikova, Olga V. ; Balandin, Taras G. ; Buryanov, Yaroslav I. ; Deyev, Sergey M. / Expression of single-chain antibody-barstar fusion in plants. In: Biochimie. 2007 ; Vol. 89, No. 1. pp. 31-38.
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