Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice

Veronika Yuskevich, Yuriy Khodarovich, German Kagarliskiy, Oleg Stremovskiy, Oksana Maksimenko, Sergey Lukash, Oleg Polanovsky, Sergey Deyev

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6 Citations (Scopus)

Abstract

A system for production of single-chain antibody in mammary glands of mice was developed on the basis of a hybrid gene constructed from the coding sequence of anti-Her2/neu single-chain antibody inserted into the first exon of the sheep beta-lactoglobulin gene. Lines of transgenic mice were obtained that expressed humanized single-chain anti-Her2/neu IgG1-like antibody in their milk. These antibodies interact with Her2/neu antigen with high affinity (K d = 0.4 nM). The expression level of the transgene depended on its integration site in the genome but not on the copy number. The transgene had no toxic effect on the mice and was stably inherited, at least for two generations. The results reveal new opportunities of producing single-chain antibodies in the milk of animals.

Original languageEnglish
Pages (from-to)628-630
Number of pages3
JournalBiochimie
Volume93
Issue number3
DOIs
Publication statusPublished - Mar 2011
Externally publishedYes

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Keywords

  • Protein expression
  • Single-chain anti-Her2/neu antibody
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yuskevich, V., Khodarovich, Y., Kagarliskiy, G., Stremovskiy, O., Maksimenko, O., Lukash, S., ... Deyev, S. (2011). Expression of humanized anti-Her2/neu single-chain IgG1-like antibody in mammary glands of transgenic mice. Biochimie, 93(3), 628-630. https://doi.org/10.1016/j.biochi.2010.12.001