Expression of G-protein coupled receptors in Escherichia coli for structural studies

L. E. Petrovskaya, A. A. Shulga, O. V. Bocharova, Ya S. Ermolyuk, E. A. Kryukova, V. V. Chupin, M. J.J. Blommers, A. S. Arseniev, M. P. Kirpichnikov

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

To elaborate a high-performance system for expression of genes of G-protein coupled receptors (GPCR), methods of direct and hybrid expression of 17 GPCR genes in Escherichia coli and selection of strains and bacteria cultivation conditions were investigated. It was established that expression of most of the target GPCR fused with the N-terminal fragment of OmpF or Mistic using media for autoinduction provides high output (up to 50 mg/liter).

Original languageEnglish
Pages (from-to)881-891
Number of pages11
JournalBiochemistry (Moscow)
Volume75
Issue number7
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Keywords

  • autoinduction
  • expression in E. coli
  • GPCR
  • Mistic
  • OmpF

ASJC Scopus subject areas

  • Biochemistry

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