Exposure to silver nanospheres leads to altered respiratory mechanics and delayed immune response in an in Vivo murine model

Danielle Botelho, Bey F. Leo, Christopher Massa, Srijata Sarkar, Terry Tetley, Kian F. Chung, Shu Chen, Mary P. Ryan, Alexandra Porter, Elena N. Atochina-Vasserman, Junfeng Zhang, Stephan Schwander, Andrew J. Gow

Research output: Contribution to journalArticle

Abstract

Here we examine the organ level toxicology of both carbon black (CB) and silver nanoparticles (AgNP). We aim to determine metal-specific effects to respiratory function, inflammation and potential interactions with lung lining fluid (LLF). C57Bl6/J male mice were intratracheally instilled with saline (control), low (0.05 μg/g) or high (0.5 μg/g) doses of either AgNP or CB 15 nm nanospheres. Lung histology, cytology, surfactant composition and function, inflammatory gene expression, and pulmonary function were measured at 1, 3, and 7 days post-exposure. Acutely, high dose CB resulted in an inflammatory response, increased neutrophilia and cytokine production, without alteration in surfactant composition or respiratory mechanics. Low dose CB had no effect. Neither low nor high dose AgNPs resulted in an acute inflammatory response, but there was an increase in work of breathing. Three days post-exposure with CB, a persistent neutrophilia was noted. High dose AgNP resulted in an elevated number of macrophages and invasion of lymphocytes. Additionally, AgNP treated mice displayed increased expression of IL1B, IL6, CCL2, and IL10. However, there were no significant changes in respiratory mechanics. At day 7, inflammation had resolved in AgNP-treated mice, but tissue stiffness and resistance were significantly decreased, which was accompanied by an increase in surfactant protein D (SP-D) content. These data demonstrate that the presence of metal alters the response of the lung to nanoparticle exposure. AgNP-surfactant interactions may alter respiratory function and result in a delayed immune response, potentially due to modified airway epithelial cell function.

Original languageEnglish
Article number213
JournalFrontiers in Pharmacology
Volume9
Issue numberMAR
DOIs
Publication statusPublished - 26 Mar 2018

Fingerprint

Nanospheres
Respiratory Mechanics
Soot
Silver
Surface-Active Agents
Lung
Nanoparticles
Metals
Pulmonary Surfactant-Associated Protein D
Work of Breathing
Inflammation
Interleukin-10
Toxicology
Cell Biology
Interleukin-6
Histology
Epithelial Cells
Macrophages
Lymphocytes
Cytokines

Keywords

  • Inflammation
  • Lung
  • Nanoparticles
  • Pulmonary function
  • Silver nanoparticles
  • Surfactant

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Exposure to silver nanospheres leads to altered respiratory mechanics and delayed immune response in an in Vivo murine model. / Botelho, Danielle; Leo, Bey F.; Massa, Christopher; Sarkar, Srijata; Tetley, Terry; Chung, Kian F.; Chen, Shu; Ryan, Mary P.; Porter, Alexandra; Atochina-Vasserman, Elena N.; Zhang, Junfeng; Schwander, Stephan; Gow, Andrew J.

In: Frontiers in Pharmacology, Vol. 9, No. MAR, 213, 26.03.2018.

Research output: Contribution to journalArticle

Botelho, D, Leo, BF, Massa, C, Sarkar, S, Tetley, T, Chung, KF, Chen, S, Ryan, MP, Porter, A, Atochina-Vasserman, EN, Zhang, J, Schwander, S & Gow, AJ 2018, 'Exposure to silver nanospheres leads to altered respiratory mechanics and delayed immune response in an in Vivo murine model', Frontiers in Pharmacology, vol. 9, no. MAR, 213. https://doi.org/10.3389/fphar.2018.00213
Botelho, Danielle ; Leo, Bey F. ; Massa, Christopher ; Sarkar, Srijata ; Tetley, Terry ; Chung, Kian F. ; Chen, Shu ; Ryan, Mary P. ; Porter, Alexandra ; Atochina-Vasserman, Elena N. ; Zhang, Junfeng ; Schwander, Stephan ; Gow, Andrew J. / Exposure to silver nanospheres leads to altered respiratory mechanics and delayed immune response in an in Vivo murine model. In: Frontiers in Pharmacology. 2018 ; Vol. 9, No. MAR.
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