Exon-Intron Structure of the fet5+ Gene of Schizosaccharomyces pombe and the Physical Mapping of the Encompassing Regions of the Genome

G. V. Shpakovski, E. N. Lebedenko

Research output: Contribution to journalArticlepeer-review

Abstract

Plasmid pYUK3 bearing the fet5+ gene of Schizosaccharomyces pombe was isolated from a genomic library of the fission yeast, and a detailed physical map of the whole genomic insert (ca. 9.6 Kbp) was constructed. The primary structure of the fet5+ gene and its flanking regions is established. The gene contains a single 45-bp intron in its distal part. A typical TATA-box (TATAAG) was found in the 5′-noncoding region ca. 50 bp upstream of the putative start of transcription, and the 3′-noncoding region contains AT-rich palindromes, which are probably involved in termination of the fet5+ transcription. A previously unidentified gene of Sz. pombe encoding a protein with some similarity to one of the transcriptional activators from the TBP (TATA-binding protein) group of SPT factors of transcription was found in the vicinity of the fet5+ gene. Taking into account that cDNA of the fet5+ gene was isolated as a suppressor of the genetic defect of nuclear RNA polymerases I - III (Bioorg. Khim., 1997, vol. 23, No. 3, pp. 234-237), this vicinity may be the first evidence of possible clustering, in the genome of the fission yeast, of genes participating in transcription regulation.

Original languageEnglish
Pages (from-to)46-47
Number of pages2
JournalBioorganicheskaya Khimiya
Volume24
Issue number1
Publication statusPublished - 1998
Externally publishedYes

Keywords

  • Eukaryotic transcription factors
  • Fet5 gene
  • Fission yeast
  • Glutamine-rich activatory domain
  • Intron

ASJC Scopus subject areas

  • Medicine(all)

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