Evidence for factor IX-independent roles for factor XIa in blood coagulation

A. Matafonov, Q. Cheng, Y. Geng, I. M. Verhamme, O. Umunakwe, E. I. Tucker, M. F. Sun, Vladimir Yurievich Serebrov, A. Gruber, D. Gailani

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    Summary: Background: Factor XIa is traditionally assigned a role in FIX activation during coagulation. However, recent evidence suggests this protease may have additional plasma substrates. Objective: To determine whether FXIa promotes thrombin generation and coagulation in plasma in the absence of FIX, and to determine whether FXI-deficiency produces an antithrombotic effect in mice independently of FIX. Methods: FXIa, FXIa variants and anti-FXIa antibodies were tested for their effects on plasma coagulation and thrombin generation in the absence of FIX, and for their effects on the activation of purified coagulation factors. Mice with combined FIX and FXI deficiency were compared with mice lacking either FIX or FXI in an arterial thrombosis model. Results: In FIX-deficient plasma, FXIa induced thrombin generation, and anti-FXIa antibodies prolonged clotting times. This process involved FXIa-mediated conversion of FX and FV to their active forms. Activation of FV by FXIa required the A3 domain on the FXIa heavy chain, whereas activation of FX did not. FX activation by FXIa, unlike FIX activation, was not a calcium-dependent process. Mice lacking both FIX and FXI were more resistant to ferric chloride-induced carotid artery occlusion than FXI-deficient or FIX-deficient mice. Conclusion: In addition to its predominant role as an activator of FIX, FXIa may contribute to coagulation by activating FX and FV. As the latter reactions do not require calcium, they may make important contributions to in vitro clotting triggered by contact activation. The reactions may be relevant to FXIa's roles in hemostasis and in promoting thrombosis.

    Original languageEnglish
    Pages (from-to)2118-2127
    Number of pages10
    JournalJournal of Thrombosis and Haemostasis
    Volume11
    Issue number12
    DOIs
    Publication statusPublished - Dec 2013

    Fingerprint

    Factor XIa
    Factor IX
    Blood Coagulation
    Thrombin
    Anti-Idiotypic Antibodies
    Thrombosis
    Calcium
    Blood Coagulation Factors
    Hemostasis
    Carotid Arteries
    Peptide Hydrolases

    Keywords

    • Factor IX
    • Factor V
    • Factor X
    • Factor XI
    • Factor XIa

    ASJC Scopus subject areas

    • Hematology
    • Medicine(all)

    Cite this

    Matafonov, A., Cheng, Q., Geng, Y., Verhamme, I. M., Umunakwe, O., Tucker, E. I., ... Gailani, D. (2013). Evidence for factor IX-independent roles for factor XIa in blood coagulation. Journal of Thrombosis and Haemostasis, 11(12), 2118-2127. https://doi.org/10.1111/jth.12435

    Evidence for factor IX-independent roles for factor XIa in blood coagulation. / Matafonov, A.; Cheng, Q.; Geng, Y.; Verhamme, I. M.; Umunakwe, O.; Tucker, E. I.; Sun, M. F.; Serebrov, Vladimir Yurievich; Gruber, A.; Gailani, D.

    In: Journal of Thrombosis and Haemostasis, Vol. 11, No. 12, 12.2013, p. 2118-2127.

    Research output: Contribution to journalArticle

    Matafonov, A, Cheng, Q, Geng, Y, Verhamme, IM, Umunakwe, O, Tucker, EI, Sun, MF, Serebrov, VY, Gruber, A & Gailani, D 2013, 'Evidence for factor IX-independent roles for factor XIa in blood coagulation', Journal of Thrombosis and Haemostasis, vol. 11, no. 12, pp. 2118-2127. https://doi.org/10.1111/jth.12435
    Matafonov A, Cheng Q, Geng Y, Verhamme IM, Umunakwe O, Tucker EI et al. Evidence for factor IX-independent roles for factor XIa in blood coagulation. Journal of Thrombosis and Haemostasis. 2013 Dec;11(12):2118-2127. https://doi.org/10.1111/jth.12435
    Matafonov, A. ; Cheng, Q. ; Geng, Y. ; Verhamme, I. M. ; Umunakwe, O. ; Tucker, E. I. ; Sun, M. F. ; Serebrov, Vladimir Yurievich ; Gruber, A. ; Gailani, D. / Evidence for factor IX-independent roles for factor XIa in blood coagulation. In: Journal of Thrombosis and Haemostasis. 2013 ; Vol. 11, No. 12. pp. 2118-2127.
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    abstract = "Summary: Background: Factor XIa is traditionally assigned a role in FIX activation during coagulation. However, recent evidence suggests this protease may have additional plasma substrates. Objective: To determine whether FXIa promotes thrombin generation and coagulation in plasma in the absence of FIX, and to determine whether FXI-deficiency produces an antithrombotic effect in mice independently of FIX. Methods: FXIa, FXIa variants and anti-FXIa antibodies were tested for their effects on plasma coagulation and thrombin generation in the absence of FIX, and for their effects on the activation of purified coagulation factors. Mice with combined FIX and FXI deficiency were compared with mice lacking either FIX or FXI in an arterial thrombosis model. Results: In FIX-deficient plasma, FXIa induced thrombin generation, and anti-FXIa antibodies prolonged clotting times. This process involved FXIa-mediated conversion of FX and FV to their active forms. Activation of FV by FXIa required the A3 domain on the FXIa heavy chain, whereas activation of FX did not. FX activation by FXIa, unlike FIX activation, was not a calcium-dependent process. Mice lacking both FIX and FXI were more resistant to ferric chloride-induced carotid artery occlusion than FXI-deficient or FIX-deficient mice. Conclusion: In addition to its predominant role as an activator of FIX, FXIa may contribute to coagulation by activating FX and FV. As the latter reactions do not require calcium, they may make important contributions to in vitro clotting triggered by contact activation. The reactions may be relevant to FXIa's roles in hemostasis and in promoting thrombosis.",
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    T1 - Evidence for factor IX-independent roles for factor XIa in blood coagulation

    AU - Matafonov, A.

    AU - Cheng, Q.

    AU - Geng, Y.

    AU - Verhamme, I. M.

    AU - Umunakwe, O.

    AU - Tucker, E. I.

    AU - Sun, M. F.

    AU - Serebrov, Vladimir Yurievich

    AU - Gruber, A.

    AU - Gailani, D.

    PY - 2013/12

    Y1 - 2013/12

    N2 - Summary: Background: Factor XIa is traditionally assigned a role in FIX activation during coagulation. However, recent evidence suggests this protease may have additional plasma substrates. Objective: To determine whether FXIa promotes thrombin generation and coagulation in plasma in the absence of FIX, and to determine whether FXI-deficiency produces an antithrombotic effect in mice independently of FIX. Methods: FXIa, FXIa variants and anti-FXIa antibodies were tested for their effects on plasma coagulation and thrombin generation in the absence of FIX, and for their effects on the activation of purified coagulation factors. Mice with combined FIX and FXI deficiency were compared with mice lacking either FIX or FXI in an arterial thrombosis model. Results: In FIX-deficient plasma, FXIa induced thrombin generation, and anti-FXIa antibodies prolonged clotting times. This process involved FXIa-mediated conversion of FX and FV to their active forms. Activation of FV by FXIa required the A3 domain on the FXIa heavy chain, whereas activation of FX did not. FX activation by FXIa, unlike FIX activation, was not a calcium-dependent process. Mice lacking both FIX and FXI were more resistant to ferric chloride-induced carotid artery occlusion than FXI-deficient or FIX-deficient mice. Conclusion: In addition to its predominant role as an activator of FIX, FXIa may contribute to coagulation by activating FX and FV. As the latter reactions do not require calcium, they may make important contributions to in vitro clotting triggered by contact activation. The reactions may be relevant to FXIa's roles in hemostasis and in promoting thrombosis.

    AB - Summary: Background: Factor XIa is traditionally assigned a role in FIX activation during coagulation. However, recent evidence suggests this protease may have additional plasma substrates. Objective: To determine whether FXIa promotes thrombin generation and coagulation in plasma in the absence of FIX, and to determine whether FXI-deficiency produces an antithrombotic effect in mice independently of FIX. Methods: FXIa, FXIa variants and anti-FXIa antibodies were tested for their effects on plasma coagulation and thrombin generation in the absence of FIX, and for their effects on the activation of purified coagulation factors. Mice with combined FIX and FXI deficiency were compared with mice lacking either FIX or FXI in an arterial thrombosis model. Results: In FIX-deficient plasma, FXIa induced thrombin generation, and anti-FXIa antibodies prolonged clotting times. This process involved FXIa-mediated conversion of FX and FV to their active forms. Activation of FV by FXIa required the A3 domain on the FXIa heavy chain, whereas activation of FX did not. FX activation by FXIa, unlike FIX activation, was not a calcium-dependent process. Mice lacking both FIX and FXI were more resistant to ferric chloride-induced carotid artery occlusion than FXI-deficient or FIX-deficient mice. Conclusion: In addition to its predominant role as an activator of FIX, FXIa may contribute to coagulation by activating FX and FV. As the latter reactions do not require calcium, they may make important contributions to in vitro clotting triggered by contact activation. The reactions may be relevant to FXIa's roles in hemostasis and in promoting thrombosis.

    KW - Factor IX

    KW - Factor V

    KW - Factor X

    KW - Factor XI

    KW - Factor XIa

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