Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Matteo Grattieri, Sofia Babanova, Carlo Santoro, Edoardo Guerrini, Stefano Pm Trasatti, Pierangela Cristiani, Massimiliano Bestetti, Plamen Atanassov

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalElectroanalysis
Volume27
Issue number2
DOIs
Publication statusPublished - 1 Feb 2015
Externally publishedYes

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Keywords

  • Bilirubin oxidase
  • Biocathode
  • Biofilm
  • Biosensor
  • MFC
  • Oxygen measurement

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Grattieri, M., Babanova, S., Santoro, C., Guerrini, E., Trasatti, S. P., Cristiani, P., Bestetti, M., & Atanassov, P. (2015). Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. Electroanalysis, 27(2), 327-335. https://doi.org/10.1002/elan.201400543