Abstract
A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
| Original language | English |
|---|---|
| Pages (from-to) | 327-335 |
| Number of pages | 9 |
| Journal | Electroanalysis |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Feb 2015 |
| Externally published | Yes |
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Keywords
- Bilirubin oxidase
- Biocathode
- Biofilm
- Biosensor
- MFC
- Oxygen measurement
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
Cite this
Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. / Grattieri, Matteo; Babanova, Sofia; Santoro, Carlo; Guerrini, Edoardo; Trasatti, Stefano Pm; Cristiani, Pierangela; Bestetti, Massimiliano; Atanassov, Plamen.
In: Electroanalysis, Vol. 27, No. 2, 01.02.2015, p. 327-335.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis
AU - Grattieri, Matteo
AU - Babanova, Sofia
AU - Santoro, Carlo
AU - Guerrini, Edoardo
AU - Trasatti, Stefano Pm
AU - Cristiani, Pierangela
AU - Bestetti, Massimiliano
AU - Atanassov, Plamen
PY - 2015/2/1
Y1 - 2015/2/1
N2 - A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
AB - A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
KW - Bilirubin oxidase
KW - Biocathode
KW - Biofilm
KW - Biosensor
KW - MFC
KW - Oxygen measurement
UR - http://www.scopus.com/inward/record.url?scp=84931578862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84931578862&partnerID=8YFLogxK
U2 - 10.1002/elan.201400543
DO - 10.1002/elan.201400543
M3 - Article
AN - SCOPUS:84931578862
VL - 27
SP - 327
EP - 335
JO - Macromolecular Chemistry and Physics
JF - Macromolecular Chemistry and Physics
SN - 1040-0397
IS - 2
ER -