Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Matteo Grattieri, Sofia Babanova, Carlo Santoro, Edoardo Guerrini, Stefano Pm Trasatti, Pierangela Cristiani, Massimiliano Bestetti, Plamen Atanassov

Research output: Contribution to journal › Article

13 Citations (Scopus)

Abstract

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL^-1 of O₂ in the bulk solution, decreasing to 0mgL^-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

Original language	English
Pages (from-to)	327-335
Number of pages	9
Journal	Electroanalysis
Volume	27
Issue number	2
DOIs	https://doi.org/10.1002/elan.201400543
Publication status	Published - 1 Feb 2015
Externally published	Yes

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Keywords

Bilirubin oxidase
Biocathode
Biofilm
Biosensor
MFC
Oxygen measurement

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

Cite this

Grattieri, M., Babanova, S., Santoro, C., Guerrini, E., Trasatti, S. P., Cristiani, P., Bestetti, M., & Atanassov, P. (2015). Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. Electroanalysis, 27(2), 327-335. https://doi.org/10.1002/elan.201400543

Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. / Grattieri, Matteo; Babanova, Sofia; Santoro, Carlo; Guerrini, Edoardo; Trasatti, Stefano Pm; Cristiani, Pierangela; Bestetti, Massimiliano; Atanassov, Plamen.

In: Electroanalysis, Vol. 27, No. 2, 01.02.2015, p. 327-335.

Research output: Contribution to journal › Article

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10.1002/elan.201400543