Abstract
A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.
| Original language | English |
|---|---|
| Pages (from-to) | 327-335 |
| Number of pages | 9 |
| Journal | Electroanalysis |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Feb 2015 |
| Externally published | Yes |
Keywords
- Bilirubin oxidase
- Biocathode
- Biofilm
- Biosensor
- MFC
- Oxygen measurement
ASJC Scopus subject areas
- Analytical Chemistry
- Electrochemistry
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Cite this
Grattieri, M., Babanova, S., Santoro, C., Guerrini, E., Trasatti, S. P., Cristiani, P., Bestetti, M., & Atanassov, P. (2015). Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. Electroanalysis, 27(2), 327-335. https://doi.org/10.1002/elan.201400543