Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Abstract

A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL^-1 of O₂ in the bulk solution, decreasing to 0mgL^-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

Original language	English
Pages (from-to)	327-335
Number of pages	9
Journal	Electroanalysis
Volume	27
Issue number	2
DOIs	https://doi.org/10.1002/elan.201400543
Publication status	Published - 1 Feb 2015
Externally published	Yes

Keywords

Bilirubin oxidase
Biocathode
Biofilm
Biosensor
MFC
Oxygen measurement

ASJC Scopus subject areas

Analytical Chemistry
Electrochemistry

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10.1002/elan.201400543

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Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. / Grattieri, Matteo; Babanova, Sofia; Santoro, Carlo; Guerrini, Edoardo; Trasatti, Stefano Pm; Cristiani, Pierangela; Bestetti, Massimiliano; Atanassov, Plamen.

In: Electroanalysis, Vol. 27, No. 2, 01.02.2015, p. 327-335.

Research output: Contribution to journal › Article › peer-review

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abstract = "A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.",

keywords = "Bilirubin oxidase, Biocathode, Biofilm, Biosensor, MFC, Oxygen measurement",

author = "Matteo Grattieri and Sofia Babanova and Carlo Santoro and Edoardo Guerrini and Trasatti, {Stefano Pm} and Pierangela Cristiani and Massimiliano Bestetti and Plamen Atanassov",

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AU - Grattieri, Matteo

AU - Babanova, Sofia

AU - Santoro, Carlo

AU - Guerrini, Edoardo

AU - Trasatti, Stefano Pm

AU - Cristiani, Pierangela

AU - Bestetti, Massimiliano

AU - Atanassov, Plamen

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