Abstract
Polysaccharides based nanocomplexes have been developed for encapsulation, controlled delivery and to enhance the phototoxicity of the photosensitizer 5-aminolevulinic acid for application in photodynamic therapy. The nanocomplexes were prepared by coacervation in a solvent free environment using chitosan as polycation while alginic and polygalacturonic acid as polyanions. The complexes showed average dimension in the range 90–120 nm, good stability in simulated physiological media and high drug encapsulation efficiency, up to 800 μg per mg of carrier. Release studies demonstrate the possibility to tune the overall release rate and the intensity of the initial burst by changing the external pH. Cytotoxicity and photocytotoxicity tests confirmed the not toxicity of the used polysaccharides. Cell viability results confirmed the improvement of 5-aminolevulinic acid phototoxicity when loaded into the carrier compared to the free form. No effect of the irradiation on the nanocomplexes structure and on the release kinetics of the drug was observed. The results demonstrate that the prepared formulations have suitable properties for future application in photodynamic therapy and to ameliorate the therapeutic efficacy and overcome the side-effects related to the use of the photosensitizer 5-aminolevulinic acid.
| Original language | English |
|---|---|
| Pages (from-to) | 226-234 |
| Number of pages | 9 |
| Journal | Journal of Photochemistry and Photobiology B: Biology |
| Volume | 175 |
| DOIs | |
| Publication status | Published - 1 Oct 2017 |
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Keywords
- 5-Aminolevulinic acid
- Controlled delivery chitosan
- Nanoparticles
- Photodynamic therapy
- Polysaccharides
ASJC Scopus subject areas
- Radiation
- Radiological and Ultrasound Technology
- Biophysics
- Radiology Nuclear Medicine and imaging
Cite this
Enhancement of 5-aminolevulinic acid phototoxicity by encapsulation in polysaccharides based nanocomplexes for photodynamic therapy application. / Di Martino, Antonio; Pavelkova, Alena; Postnikov, Pavel S.; Sedlarik, Vladimir.
In: Journal of Photochemistry and Photobiology B: Biology, Vol. 175, 01.10.2017, p. 226-234.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancement of 5-aminolevulinic acid phototoxicity by encapsulation in polysaccharides based nanocomplexes for photodynamic therapy application
AU - Di Martino, Antonio
AU - Pavelkova, Alena
AU - Postnikov, Pavel S.
AU - Sedlarik, Vladimir
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Polysaccharides based nanocomplexes have been developed for encapsulation, controlled delivery and to enhance the phototoxicity of the photosensitizer 5-aminolevulinic acid for application in photodynamic therapy. The nanocomplexes were prepared by coacervation in a solvent free environment using chitosan as polycation while alginic and polygalacturonic acid as polyanions. The complexes showed average dimension in the range 90–120 nm, good stability in simulated physiological media and high drug encapsulation efficiency, up to 800 μg per mg of carrier. Release studies demonstrate the possibility to tune the overall release rate and the intensity of the initial burst by changing the external pH. Cytotoxicity and photocytotoxicity tests confirmed the not toxicity of the used polysaccharides. Cell viability results confirmed the improvement of 5-aminolevulinic acid phototoxicity when loaded into the carrier compared to the free form. No effect of the irradiation on the nanocomplexes structure and on the release kinetics of the drug was observed. The results demonstrate that the prepared formulations have suitable properties for future application in photodynamic therapy and to ameliorate the therapeutic efficacy and overcome the side-effects related to the use of the photosensitizer 5-aminolevulinic acid.
AB - Polysaccharides based nanocomplexes have been developed for encapsulation, controlled delivery and to enhance the phototoxicity of the photosensitizer 5-aminolevulinic acid for application in photodynamic therapy. The nanocomplexes were prepared by coacervation in a solvent free environment using chitosan as polycation while alginic and polygalacturonic acid as polyanions. The complexes showed average dimension in the range 90–120 nm, good stability in simulated physiological media and high drug encapsulation efficiency, up to 800 μg per mg of carrier. Release studies demonstrate the possibility to tune the overall release rate and the intensity of the initial burst by changing the external pH. Cytotoxicity and photocytotoxicity tests confirmed the not toxicity of the used polysaccharides. Cell viability results confirmed the improvement of 5-aminolevulinic acid phototoxicity when loaded into the carrier compared to the free form. No effect of the irradiation on the nanocomplexes structure and on the release kinetics of the drug was observed. The results demonstrate that the prepared formulations have suitable properties for future application in photodynamic therapy and to ameliorate the therapeutic efficacy and overcome the side-effects related to the use of the photosensitizer 5-aminolevulinic acid.
KW - 5-Aminolevulinic acid
KW - Controlled delivery chitosan
KW - Nanoparticles
KW - Photodynamic therapy
KW - Polysaccharides
UR - http://www.scopus.com/inward/record.url?scp=85029289691&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029289691&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2017.08.010
DO - 10.1016/j.jphotobiol.2017.08.010
M3 - Article
AN - SCOPUS:85029289691
VL - 175
SP - 226
EP - 234
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
ER -