Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme

V. R. Muzykantov, E. A. Puchnina, Elena Nikolaevna Atochina, H. Hiemish, M. A. Slinkin, F. E. Meertsuk, S. M. Danilov

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The biodistribution of radiolabeled monoclonal antibody (Mab) to angiotensin-converting enzyme (ACE) was examined in normal and endotoxin-treated rats. Endotoxin administration at a dose of 4 mg/kg induced mild or middle pulmonary edema. The ACE activity in lung homogenate remained virtually unchanged, while the activity of serum ACE increased 15 hr after endotoxin infusion. In normal rats, anti-ACE Mab accumulates specifically in the lung after i.v. injection. Endotoxin injection induces reduction of specific pulmonary uptake of this antibody. Even in nonedematous endotoxemia, the accumulation of anti-ACE Mab antibody (Mab 9B9) decreased from 19.02 to 11.91% of ID/g of tissue without any change in accumulation of control nonspecific IgG. The antibody distribution in other organs and its blood level were almost the same as in the control. In a case of endotoxemia accompanied by increased microvascular permeability, the lung accumulation of Mab 9B9 was reduced to 9.17% of ID/g of tissue, while the accumulation of nonspecific IgG increased to 1.44% versus 0.89% in the control.

Original languageEnglish
Pages (from-to)453-460
Number of pages8
JournalJournal of Nuclear Medicine
Volume32
Issue number3
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Peptidyl-Dipeptidase A
Endotoxins
Monoclonal Antibodies
Lung
Endotoxemia
Antibodies
Immunoglobulin G
Injections
Capillary Permeability
Pulmonary Edema
Serum

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Muzykantov, V. R., Puchnina, E. A., Atochina, E. N., Hiemish, H., Slinkin, M. A., Meertsuk, F. E., & Danilov, S. M. (1991). Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme. Journal of Nuclear Medicine, 32(3), 453-460.

Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme. / Muzykantov, V. R.; Puchnina, E. A.; Atochina, Elena Nikolaevna; Hiemish, H.; Slinkin, M. A.; Meertsuk, F. E.; Danilov, S. M.

In: Journal of Nuclear Medicine, Vol. 32, No. 3, 1991, p. 453-460.

Research output: Contribution to journalArticle

Muzykantov, VR, Puchnina, EA, Atochina, EN, Hiemish, H, Slinkin, MA, Meertsuk, FE & Danilov, SM 1991, 'Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme', Journal of Nuclear Medicine, vol. 32, no. 3, pp. 453-460.
Muzykantov, V. R. ; Puchnina, E. A. ; Atochina, Elena Nikolaevna ; Hiemish, H. ; Slinkin, M. A. ; Meertsuk, F. E. ; Danilov, S. M. / Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme. In: Journal of Nuclear Medicine. 1991 ; Vol. 32, No. 3. pp. 453-460.
@article{dae934ba22fc47bead71304ab844d541,
title = "Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme",
abstract = "The biodistribution of radiolabeled monoclonal antibody (Mab) to angiotensin-converting enzyme (ACE) was examined in normal and endotoxin-treated rats. Endotoxin administration at a dose of 4 mg/kg induced mild or middle pulmonary edema. The ACE activity in lung homogenate remained virtually unchanged, while the activity of serum ACE increased 15 hr after endotoxin infusion. In normal rats, anti-ACE Mab accumulates specifically in the lung after i.v. injection. Endotoxin injection induces reduction of specific pulmonary uptake of this antibody. Even in nonedematous endotoxemia, the accumulation of anti-ACE Mab antibody (Mab 9B9) decreased from 19.02 to 11.91{\%} of ID/g of tissue without any change in accumulation of control nonspecific IgG. The antibody distribution in other organs and its blood level were almost the same as in the control. In a case of endotoxemia accompanied by increased microvascular permeability, the lung accumulation of Mab 9B9 was reduced to 9.17{\%} of ID/g of tissue, while the accumulation of nonspecific IgG increased to 1.44{\%} versus 0.89{\%} in the control.",
author = "Muzykantov, {V. R.} and Puchnina, {E. A.} and Atochina, {Elena Nikolaevna} and H. Hiemish and Slinkin, {M. A.} and Meertsuk, {F. E.} and Danilov, {S. M.}",
year = "1991",
language = "English",
volume = "32",
pages = "453--460",
journal = "Journal of Nuclear Medicine",
issn = "0161-5505",
publisher = "Society of Nuclear Medicine Inc.",
number = "3",

}

TY - JOUR

T1 - Endotoxin reduces specific pulmonary uptake of radiolabeled monoclonal antibody to angiotensin-converting enzyme

AU - Muzykantov, V. R.

AU - Puchnina, E. A.

AU - Atochina, Elena Nikolaevna

AU - Hiemish, H.

AU - Slinkin, M. A.

AU - Meertsuk, F. E.

AU - Danilov, S. M.

PY - 1991

Y1 - 1991

N2 - The biodistribution of radiolabeled monoclonal antibody (Mab) to angiotensin-converting enzyme (ACE) was examined in normal and endotoxin-treated rats. Endotoxin administration at a dose of 4 mg/kg induced mild or middle pulmonary edema. The ACE activity in lung homogenate remained virtually unchanged, while the activity of serum ACE increased 15 hr after endotoxin infusion. In normal rats, anti-ACE Mab accumulates specifically in the lung after i.v. injection. Endotoxin injection induces reduction of specific pulmonary uptake of this antibody. Even in nonedematous endotoxemia, the accumulation of anti-ACE Mab antibody (Mab 9B9) decreased from 19.02 to 11.91% of ID/g of tissue without any change in accumulation of control nonspecific IgG. The antibody distribution in other organs and its blood level were almost the same as in the control. In a case of endotoxemia accompanied by increased microvascular permeability, the lung accumulation of Mab 9B9 was reduced to 9.17% of ID/g of tissue, while the accumulation of nonspecific IgG increased to 1.44% versus 0.89% in the control.

AB - The biodistribution of radiolabeled monoclonal antibody (Mab) to angiotensin-converting enzyme (ACE) was examined in normal and endotoxin-treated rats. Endotoxin administration at a dose of 4 mg/kg induced mild or middle pulmonary edema. The ACE activity in lung homogenate remained virtually unchanged, while the activity of serum ACE increased 15 hr after endotoxin infusion. In normal rats, anti-ACE Mab accumulates specifically in the lung after i.v. injection. Endotoxin injection induces reduction of specific pulmonary uptake of this antibody. Even in nonedematous endotoxemia, the accumulation of anti-ACE Mab antibody (Mab 9B9) decreased from 19.02 to 11.91% of ID/g of tissue without any change in accumulation of control nonspecific IgG. The antibody distribution in other organs and its blood level were almost the same as in the control. In a case of endotoxemia accompanied by increased microvascular permeability, the lung accumulation of Mab 9B9 was reduced to 9.17% of ID/g of tissue, while the accumulation of nonspecific IgG increased to 1.44% versus 0.89% in the control.

UR - http://www.scopus.com/inward/record.url?scp=0026093203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026093203&partnerID=8YFLogxK

M3 - Article

VL - 32

SP - 453

EP - 460

JO - Journal of Nuclear Medicine

JF - Journal of Nuclear Medicine

SN - 0161-5505

IS - 3

ER -