Endothelial nitric oxide synthase transgenic models of endothelial dysfunction

Dmitriy N. Atochin, Paul L. Huang

Research output: Contribution to journalReview article

74 Citations (Scopus)

Abstract

Endothelial production of nitric oxide is critical to the regulation of vascular responses, including vascular tone and regional blood flow, leukocyte-endothelial interactions, platelet adhesion and aggregation, and vascular smooth muscle cell proliferation. A relative deficiency in the amount of bioavailable vascular NO results in endothelial dysfunction, with conditions that are conducive to the development of atherosclerosis: thrombosis, inflammation, neointimal proliferation, and vasoconstriction. This review focuses on mouse models of endothelial dysfunction caused by direct genetic modification of the endothelial nitric oxide synthase (eNOS) gene. We first describe the cardiovascular phenotypes of eNOS knockout mice, which are a model of total eNOS gene deficiency and thus the ultimate model of endothelial dysfunction. We then describe S1177A and S1177D eNOS mutant mice as mouse models with altered eNOS phosphorylation and therefore varying degrees of endothelial dysfunction. These include transgenic mice that carry the eNOS S1177A and S1177D transgenes, as well as knockin mice in which the endogenous eNOS gene has been mutated to carry the S1177A and S1177D mutations. Together, eNOS knockout mice and eNOS S1177 mutant mice are useful tools to study the effects of total genetic deficiency of eNOS as well as varying degrees of endothelial dysfunction caused by eNOS S1177 phosphorylation.

Original languageEnglish
Pages (from-to)965-974
Number of pages10
JournalPflugers Archiv European Journal of Physiology
Volume460
Issue number6
DOIs
Publication statusPublished - Nov 2010
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type III
Blood Vessels
Phosphorylation
Genes
Knockout Mice
Regional Blood Flow
Cell proliferation
Platelets
Vasoconstriction
Transgenes
Platelet Aggregation
Vascular Smooth Muscle
Transgenic Mice
Smooth Muscle Myocytes
Muscle
Atherosclerosis
Nitric Oxide
Thrombosis
Leukocytes
Blood

Keywords

  • Cerebral circulation
  • Endothelium
  • Nitric oxide
  • Transgenic mouse
  • Vascular dysfunction

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

Cite this

Endothelial nitric oxide synthase transgenic models of endothelial dysfunction. / Atochin, Dmitriy N.; Huang, Paul L.

In: Pflugers Archiv European Journal of Physiology, Vol. 460, No. 6, 11.2010, p. 965-974.

Research output: Contribution to journalReview article

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