Abstract
Human endopeptidase 24.11 (EP) occurs in greatest abundance on terminally differentiated prostate cells; thus, loss of EP could mark dedifferentiation of prostate epithelium. To identify laboratory models that would permit continuous work oil the biochemistry and hormonal regulation of EP, we examined the well differentiated LNCaP and poorly differentiated PPC-1 human prostate cancer cell lines. Ultrastructural analysis revealed that LNCaP secretes electron-dense material that resembles the particulate matter of seminal plasma, which is associated with endopeptidase activity. LNCaP medium contained EP activity while PPC-1 medium did not. Whether the apparent deletion of EP from the PPC-1 cell line is characteristic of poorly differentiated prostate adenocarcinoma is not yet clear. However, it may be relevant to the carcinogenic process that EP can limit growth of lung small carcinomas by inactivating cell growth-promoting bombesin-like peptides. Because bombesin has been identified in aggressive human prostate cancers, loss of EP in PPC-1 could represent a necessary step in transformation to aggressive phenotype. The combination of LNCaP and PPC-1, which offers well-differentiated and poorly differentiated cancer phenotypes, appears well suited to studying the relevance of EP in prostate cancer biology.
| Original language | English |
|---|---|
| Pages (from-to) | 113-116 |
| Number of pages | 4 |
| Journal | Urological Research |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 1997 |
| Externally published | Yes |
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Keywords
- Endopeptidase 24.11
- Growth
- LNCaP
- PPC-1
- Prostate cancer
ASJC Scopus subject areas
- Urology
Cite this
Endopeptidase 24.11 activity in the human prostate cancer cell lines LNCaP and PPC-1. / Krongrad, A.; Atochina, Elena Nikolaevna; Ryan, J. V V; Roos, B. A.
In: Urological Research, Vol. 25, No. 2, 04.1997, p. 113-116.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Endopeptidase 24.11 activity in the human prostate cancer cell lines LNCaP and PPC-1
AU - Krongrad, A.
AU - Atochina, Elena Nikolaevna
AU - Ryan, J. V V
AU - Roos, B. A.
PY - 1997/4
Y1 - 1997/4
N2 - Human endopeptidase 24.11 (EP) occurs in greatest abundance on terminally differentiated prostate cells; thus, loss of EP could mark dedifferentiation of prostate epithelium. To identify laboratory models that would permit continuous work oil the biochemistry and hormonal regulation of EP, we examined the well differentiated LNCaP and poorly differentiated PPC-1 human prostate cancer cell lines. Ultrastructural analysis revealed that LNCaP secretes electron-dense material that resembles the particulate matter of seminal plasma, which is associated with endopeptidase activity. LNCaP medium contained EP activity while PPC-1 medium did not. Whether the apparent deletion of EP from the PPC-1 cell line is characteristic of poorly differentiated prostate adenocarcinoma is not yet clear. However, it may be relevant to the carcinogenic process that EP can limit growth of lung small carcinomas by inactivating cell growth-promoting bombesin-like peptides. Because bombesin has been identified in aggressive human prostate cancers, loss of EP in PPC-1 could represent a necessary step in transformation to aggressive phenotype. The combination of LNCaP and PPC-1, which offers well-differentiated and poorly differentiated cancer phenotypes, appears well suited to studying the relevance of EP in prostate cancer biology.
AB - Human endopeptidase 24.11 (EP) occurs in greatest abundance on terminally differentiated prostate cells; thus, loss of EP could mark dedifferentiation of prostate epithelium. To identify laboratory models that would permit continuous work oil the biochemistry and hormonal regulation of EP, we examined the well differentiated LNCaP and poorly differentiated PPC-1 human prostate cancer cell lines. Ultrastructural analysis revealed that LNCaP secretes electron-dense material that resembles the particulate matter of seminal plasma, which is associated with endopeptidase activity. LNCaP medium contained EP activity while PPC-1 medium did not. Whether the apparent deletion of EP from the PPC-1 cell line is characteristic of poorly differentiated prostate adenocarcinoma is not yet clear. However, it may be relevant to the carcinogenic process that EP can limit growth of lung small carcinomas by inactivating cell growth-promoting bombesin-like peptides. Because bombesin has been identified in aggressive human prostate cancers, loss of EP in PPC-1 could represent a necessary step in transformation to aggressive phenotype. The combination of LNCaP and PPC-1, which offers well-differentiated and poorly differentiated cancer phenotypes, appears well suited to studying the relevance of EP in prostate cancer biology.
KW - Endopeptidase 24.11
KW - Growth
KW - LNCaP
KW - PPC-1
KW - Prostate cancer
UR - http://www.scopus.com/inward/record.url?scp=0030904907&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030904907&partnerID=8YFLogxK
U2 - 10.1007/BF01037925
DO - 10.1007/BF01037925
M3 - Article
VL - 25
SP - 113
EP - 116
JO - Urological Research
JF - Urological Research
SN - 0300-5623
IS - 2
ER -