Abstract
Aims: To investigate electrochemical oxidability of antioxidants to reveal synergistic and antagonistic effects in mixes. Materials and Methods: Electrochemical oxidability of some widely used antioxidants, including uric acid, glutathione, trolox, ascorbic acid, gallic acid, Mexidol, and potassium fenozan, was investigated by the amperometric approach. Results: All obtained electrochemical oxidability values correlate well with antioxidant activity parameters of the same compounds measured by other methods. The measurements of the electrochemical oxidability for binary mixes of substances were tested to reveal any synergistic actions. The experimental results and calculated values overlap for various combinations of tested mixes. It testifies to the absence of interaction between them (both synergism and antagonism) in the oxidation process. Conclusions: The constants of oxidation were defined for different antioxidants and mixes by amperometric approach. Most mixes of probed compounds revealed absence of interaction between them in oxidation process. In some cases (mainly with glutathione and ascorbic acid), antagonism takes place, deteriorating effects of their joint application. Apparently, a partial reduction of glutathione by ascorbic acid leads to excess of the measured value over calculated value.
| Original language | English |
|---|---|
| Pages (from-to) | 60-65 |
| Number of pages | 6 |
| Journal | Journal of Pharmacy and Bioallied Sciences |
| Volume | 10 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Apr 2018 |
Fingerprint
Keywords
- Amperometry
- antagonism
- antioxidant
- oxidability
- synergism
ASJC Scopus subject areas
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Electrochemical oxidability of antioxidants : Synergism and antagonism in mixes. / Sazhina, Natalia; Plotnikov, Evgenii; Korotkova, Elena; Dorozhko, Elena; Voronova, Olesya.
In: Journal of Pharmacy and Bioallied Sciences, Vol. 10, No. 2, 01.04.2018, p. 60-65.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrochemical oxidability of antioxidants
T2 - Synergism and antagonism in mixes
AU - Sazhina, Natalia
AU - Plotnikov, Evgenii
AU - Korotkova, Elena
AU - Dorozhko, Elena
AU - Voronova, Olesya
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Aims: To investigate electrochemical oxidability of antioxidants to reveal synergistic and antagonistic effects in mixes. Materials and Methods: Electrochemical oxidability of some widely used antioxidants, including uric acid, glutathione, trolox, ascorbic acid, gallic acid, Mexidol, and potassium fenozan, was investigated by the amperometric approach. Results: All obtained electrochemical oxidability values correlate well with antioxidant activity parameters of the same compounds measured by other methods. The measurements of the electrochemical oxidability for binary mixes of substances were tested to reveal any synergistic actions. The experimental results and calculated values overlap for various combinations of tested mixes. It testifies to the absence of interaction between them (both synergism and antagonism) in the oxidation process. Conclusions: The constants of oxidation were defined for different antioxidants and mixes by amperometric approach. Most mixes of probed compounds revealed absence of interaction between them in oxidation process. In some cases (mainly with glutathione and ascorbic acid), antagonism takes place, deteriorating effects of their joint application. Apparently, a partial reduction of glutathione by ascorbic acid leads to excess of the measured value over calculated value.
AB - Aims: To investigate electrochemical oxidability of antioxidants to reveal synergistic and antagonistic effects in mixes. Materials and Methods: Electrochemical oxidability of some widely used antioxidants, including uric acid, glutathione, trolox, ascorbic acid, gallic acid, Mexidol, and potassium fenozan, was investigated by the amperometric approach. Results: All obtained electrochemical oxidability values correlate well with antioxidant activity parameters of the same compounds measured by other methods. The measurements of the electrochemical oxidability for binary mixes of substances were tested to reveal any synergistic actions. The experimental results and calculated values overlap for various combinations of tested mixes. It testifies to the absence of interaction between them (both synergism and antagonism) in the oxidation process. Conclusions: The constants of oxidation were defined for different antioxidants and mixes by amperometric approach. Most mixes of probed compounds revealed absence of interaction between them in oxidation process. In some cases (mainly with glutathione and ascorbic acid), antagonism takes place, deteriorating effects of their joint application. Apparently, a partial reduction of glutathione by ascorbic acid leads to excess of the measured value over calculated value.
KW - Amperometry
KW - antagonism
KW - antioxidant
KW - oxidability
KW - synergism
UR - http://www.scopus.com/inward/record.url?scp=85048346479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048346479&partnerID=8YFLogxK
U2 - 10.4103/JPBS.JPBS_203_17
DO - 10.4103/JPBS.JPBS_203_17
M3 - Article
VL - 10
SP - 60
EP - 65
JO - Journal of Pharmacy and Bioallied Sciences
JF - Journal of Pharmacy and Bioallied Sciences
SN - 0976-4879
IS - 2
ER -