Electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals

Andrea Hájková, Jiří Barek, Vlastimil Vyskočil

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A simple electrochemical DNA biosensor based on a glassy carbon electrode (GCE) was prepared by adsorbing double-stranded DNA (dsDNA) onto the GCE surface and subsequently used for the detection of dsDNA damage induced by hydroxyl radicals. Investigation of the mutual interaction between hydroxyl radicals and dsDNA was conducted using a combination of several electrochemical detection techniques: square-wave voltammetry for direct monitoring the oxidation of dsDNA bases, and cyclic voltammetry and electrochemical impedance spectroscopy as indirect electrochemical methods making use of the redox-active indicator [Fe(CN)6]4 −/3 −. Hydroxyl radicals were generated electrochemically on the surface of a boron-doped diamond electrode and chemically (via the Fenton's reaction or the auto-oxidation of Fe(II)). The extent of dsDNA damage by electrochemically generated hydroxyl radicals depended on the current density applied to the generating electrode: by applying 5, 10, and 50 mA cm− 2, selected relative biosensor responses decreased after 3 min incubation from 100% to 38%, 27%, and 3%, respectively. Chemically generated hydroxyl radicals caused less pronounced dsDNA damage, and their damaging activity depended on the form of Fe(II) ions: decreases to 49% (Fenton's reaction; Fe(II) complexed with EDTA) and 33% (auto-oxidation of Fe(II); Fe(II) complexed with dsDNA) were observed after 10 min incubation.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalBioelectrochemistry
Volume116
DOIs
Publication statusPublished - 1 Aug 2017
Externally publishedYes

Fingerprint

hydroxyl radicals
Biosensing Techniques
bioinstrumentation
Biosensors
Hydroxyl Radical
DNA
deoxyribonucleic acid
damage
Electrodes
DNA Damage
electrodes
glassy carbon
Glassy carbon
Oxidation
oxidation
Carbon
Electrochemical Techniques
Dielectric Spectroscopy
Diamond
Boron

Keywords

  • Auto-oxidation
  • Boron-doped diamond electrode
  • Electrochemical DNA biosensor
  • Fenton's reaction
  • Glassy carbon electrode
  • Hydroxyl radicals

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

Cite this

Electrochemical DNA biosensor for detection of DNA damage induced by hydroxyl radicals. / Hájková, Andrea; Barek, Jiří; Vyskočil, Vlastimil.

In: Bioelectrochemistry, Vol. 116, 01.08.2017, p. 1-9.

Research output: Contribution to journalArticle

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