Electrochemical determination of heparin in pharmaceuticals with using malachite green

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Abstract

In this paper for determination of heparin in pharmaceuticals by constant-current sweep voltammetry on the mercury-film electrode (MFE) we used cationic malachite green (MG) dye which is capable to form a complex with heparin. In the 0.05 M potassium tetraoxalate (KH3C4O8 · 2H2O) buffer solution with pH 1.65 MG had a well-defined peak of electroreduction at -0.46 V (vs. CSE). During the study of electrochemical properties of heparin in complex with MG in cathodic potential range from 0.0 V to -1.3 V on MFE besides the signal from the dye at E = - 0.46 V the second signal at E = –0.87 V was found. Increasing the concentration of heparin in the solution led to decrease of signal intensity at E = -0.87 V. Optimum conditions for the electrochemical determination of heparin in dosage form with using MG were picked up. Influence of auxiliary components of heparin pharmacological solution on electroreduction of the complex MG-Hep was investigated. Under optimal conditions the decrease of reduction peak currents at E = -0.87 V was proportional to heparin concentration in the range from 3.9 · 10-4 mg/ml to 3.9 · 10-3 mg/ml.

Original languageEnglish
Title of host publicationAdvanced Materials Research
PublisherTrans Tech Publications Ltd
Pages292-296
Number of pages5
Volume1040
ISBN (Print)9783038352648
DOIs
Publication statusPublished - 2014
EventInternational Conference for Young Scientists “High Technology: Research and Applications 2014”, HTRA 2014 - Tomsk, Russian Federation
Duration: 26 Mar 201428 Mar 2014

Publication series

NameAdvanced Materials Research
Volume1040
ISSN (Print)10226680
ISSN (Electronic)16628985

Other

OtherInternational Conference for Young Scientists “High Technology: Research and Applications 2014”, HTRA 2014
CountryRussian Federation
CityTomsk
Period26.3.1428.3.14

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Keywords

  • Electroreduction
  • Heparin
  • Malachite green
  • Mercury-film electrode
  • Voltammetry

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Vishenkova, D. A., Korotkova, E. I., & Dorozhko, E. V. (2014). Electrochemical determination of heparin in pharmaceuticals with using malachite green. In Advanced Materials Research (Vol. 1040, pp. 292-296). (Advanced Materials Research; Vol. 1040). Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/AMR.1040.292