Abstract
Nitric oxide is a key regulator of diverse physiological processes that include vasoregulation, platelet and neutrophil adherence, and immune defense. The detection and quantification of nitric oxide has been accomplishd by a variety of methodologies. In this paper we desribe methodologies to detect nitric oxide in solution using an electrochemical detector and provide examples of nitric oxide detection in biological systems including data from plasma and cell suspensions. The experiments outlined show that electrochemical detection is suitable for detecting and quantifying nitric oxide within biological samples and buffers. Potential pitfalls and necessary controls are discussed. The use of electrochemical detection was also found to be useful in determining the flux of nitric oxide produced by donor species in different biological buffers.
| Original language | English |
|---|---|
| Pages (from-to) | 146-154 |
| Number of pages | 9 |
| Journal | Microchemical Journal |
| Volume | 56 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 1997 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Spectroscopy
Cite this
Electrochemical detection of nitric oxide in biological systems. / Gow, Andrew J.; Thom, Stephen R.; Brass, Clifford; Ischiropoulos, Harry.
In: Microchemical Journal, Vol. 56, No. 2, 06.1997, p. 146-154.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrochemical detection of nitric oxide in biological systems
AU - Gow, Andrew J.
AU - Thom, Stephen R.
AU - Brass, Clifford
AU - Ischiropoulos, Harry
PY - 1997/6
Y1 - 1997/6
N2 - Nitric oxide is a key regulator of diverse physiological processes that include vasoregulation, platelet and neutrophil adherence, and immune defense. The detection and quantification of nitric oxide has been accomplishd by a variety of methodologies. In this paper we desribe methodologies to detect nitric oxide in solution using an electrochemical detector and provide examples of nitric oxide detection in biological systems including data from plasma and cell suspensions. The experiments outlined show that electrochemical detection is suitable for detecting and quantifying nitric oxide within biological samples and buffers. Potential pitfalls and necessary controls are discussed. The use of electrochemical detection was also found to be useful in determining the flux of nitric oxide produced by donor species in different biological buffers.
AB - Nitric oxide is a key regulator of diverse physiological processes that include vasoregulation, platelet and neutrophil adherence, and immune defense. The detection and quantification of nitric oxide has been accomplishd by a variety of methodologies. In this paper we desribe methodologies to detect nitric oxide in solution using an electrochemical detector and provide examples of nitric oxide detection in biological systems including data from plasma and cell suspensions. The experiments outlined show that electrochemical detection is suitable for detecting and quantifying nitric oxide within biological samples and buffers. Potential pitfalls and necessary controls are discussed. The use of electrochemical detection was also found to be useful in determining the flux of nitric oxide produced by donor species in different biological buffers.
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UR - http://www.scopus.com/inward/citedby.url?scp=0001027546&partnerID=8YFLogxK
U2 - 10.1006/mchj.1996.1443
DO - 10.1006/mchj.1996.1443
M3 - Article
VL - 56
SP - 146
EP - 154
JO - Microchemical Journal
JF - Microchemical Journal
SN - 0026-265X
IS - 2
ER -