Electrochemical detection of nitric oxide in biological systems

Andrew J. Gow, Stephen R. Thom, Clifford Brass, Harry Ischiropoulos

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)


    Nitric oxide is a key regulator of diverse physiological processes that include vasoregulation, platelet and neutrophil adherence, and immune defense. The detection and quantification of nitric oxide has been accomplishd by a variety of methodologies. In this paper we desribe methodologies to detect nitric oxide in solution using an electrochemical detector and provide examples of nitric oxide detection in biological systems including data from plasma and cell suspensions. The experiments outlined show that electrochemical detection is suitable for detecting and quantifying nitric oxide within biological samples and buffers. Potential pitfalls and necessary controls are discussed. The use of electrochemical detection was also found to be useful in determining the flux of nitric oxide produced by donor species in different biological buffers.

    Original languageEnglish
    Pages (from-to)146-154
    Number of pages9
    JournalMicrochemical Journal
    Issue number2
    Publication statusPublished - Jun 1997

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Spectroscopy

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