Electrochemical biosensors based on enzymatic reactor of silver solid amalgam powder for measurements in flow systems

Oksana Josypčuk, Jiří Barek, Bohdan Josypčuk

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An enzymatic reactor based on silver solid amalgam powder was suggested as the main part of biosensors in flow systems for the first time. Biosensors were tested with following enzymes: ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. The current response of each biosensor was optimized with respect to the detection potential, flow rate, the injection and reactor volume. Relative standard deviation for detection with the studied enzymes was found to be in the range of 0.81-2.1%. The biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablets Celaskon.

Original languageEnglish
Pages (from-to)1729-1738
Number of pages10
JournalElectroanalysis
Volume26
Issue number8
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Amalgam powder
  • Amperometry
  • Electrochemical biosensors
  • Enzymatic reactor
  • Flow systems

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

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