Efficiency of Bioluminescence Resonance Energy Transfer in the NanoLuc-miniSOG-Furimazine System

E. I. Shramova, S. M. Deyev, G. M. Proshkina

Research output: Contribution to journalArticlepeer-review

Abstract

Photodynamic therapy (PDT) is a clinically approved, minimally invasive method for tumor destruction in the presence of a photosensitizer (PS), oxygen, and a light source. The main obstacle for the PDT treatment of deep tumors is the problem of the excitation light delivery inside the body without reduction of light intensity. An internal light source based on a bioluminescence system NanoLuc-furimazine can be considered as an alternative to an external light source. This system can be successfully used to excite a protein photosensitizer miniSOG and induce its phototoxicity in cancer cells as a result of bioluminescent resonance energy transfer. In this paper, the effectiveness of bioluminescent resonance energy transfer in the NanoLuc-miniSOG-furimazine system in living cells was studied. The efficiency of the energy transfer in this system was found to be independent of the furimazine concentration, while intracellular localization of the NanoLuc-miniSOG hybrid protein was found to be a crucial factor.

Original languageEnglish
Pages (from-to)755-758
Number of pages4
JournalRussian Journal of Bioorganic Chemistry
Volume44
Issue number6
DOIs
Publication statusPublished - 1 Nov 2018
Externally publishedYes

Keywords

  • bioluminescence resonance energy transfer
  • furimazine
  • NanoLuc luciferase

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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