Effects of neuroglobin overexpression on acute brain injury and long-term outcomes after focal cerebral ischemia

Xiaoying Wang, Jianxiang Liu, Haihao Zhu, Emiri Tejima, Kiyoshi Tsuji, Yoshihiro Murata, Dmitriy N. Atochin, Paul L. Huang, Chenggang Zhang, Eng H. Lo

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122 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE: Emerging data suggest that neuroglobin (Ngb) may protect against hypoxic/ischemic neuronal insults. However, the underlying mechanisms in vivo and implications for long-term outcomes are still not well understood. METHODS: Using our newly created Ngb overexpressing transgenic (Ngb-Tg) mice, we measured brain infarction on day 1 and day 14 after transient focal cerebral ischemia and performed neurobehavioral assessments in sensorimotor deficits on days 1, 3, 7, and 14. To test the hypothesis that Ngb may play a role in reducing oxidative stress after stroke, intracellular malondialdehyde levels were measured and compared in Ngb-Tg and wild-type mice. RESULTS: Increased Ngb mRNA and protein levels were identified in Ngb-Tg brains. Malondialdehyde levels in ischemic hemispheres of Ngb-Tg were significantly reduced compared with wild-type controls at 8 hours and 22 hours after transient focal cerebral ischemia. Compared with wild-type controls, brain infarction volumes 1 day and 14 days after transient focal cerebral ischemia were significantly reduced in Ngb-Tg mice. However, there were no significant improvements in sensorimotor deficits for up to 14 days after stroke in Ngb-Tg mice compared with wild-type controls. CONCLUSIONS: Ngb reduces tissue infarction and markers of oxidative stress after stroke. Tissue protection by overexpressing Ngb can be sustained for up to 2 weeks.

Original languageEnglish
Pages (from-to)1869-1874
Number of pages6
JournalStroke
Volume39
Issue number6
DOIs
Publication statusPublished - 1 Jun 2008
Externally publishedYes

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Keywords

  • Neuroglobin
  • Nuroprotection
  • Oxidative stress
  • Stroke

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialised Nursing
  • Medicine(all)

Cite this

Wang, X., Liu, J., Zhu, H., Tejima, E., Tsuji, K., Murata, Y., Atochin, D. N., Huang, P. L., Zhang, C., & Lo, E. H. (2008). Effects of neuroglobin overexpression on acute brain injury and long-term outcomes after focal cerebral ischemia. Stroke, 39(6), 1869-1874. https://doi.org/10.1161/STROKEAHA.107.506022