Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide: a Screening Study

E. S. Trofimova, M. V. Zykova, A. A. Ligacheva, E. Yu Sherstoboev, V. V. Zhdanov, M. V. Belousov, M. S. Yusubov, S. V. Krivoshchekov, M. G. Danilets, A. M. Dygai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A screening study of biological activity of native humic acids isolated from peat was performed; several physical and chemical parameters of their structures were studied by UV- and infrared spectroscopy. Spectroscopy yielded similar shape of light absorption curves of humic acids of different origin, which can reflect similarity of general structural principles of these substances. Alkaline humic acids have more developed system of polyconjugation, while molecular structures of pyrophosphate humic acids were characterized by higher aromaticity and condensation indexes. Biological activity of the studied humic acids was assessed by NO-stimulating capacity during their culturing with murine peritoneal macrophages in a wide concentration range. It was shown that due to dose-dependent enhancement of NO production humic acids can change the functional state of macrophages towards development of pro-inflammatory properties. These changes were associated with high activity of humic acids isolated by pyrophosphate extraction, which allows considering effects of isolation method on biological activity.

Original languageEnglish
Pages (from-to)687-692
Number of pages6
JournalBulletin of Experimental Biology and Medicine
Volume161
Issue number5
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

Humic Substances
Peat
Screening
Nitric Oxide
Soil
Bioactivity
Macrophages
Spectrum Analysis
Peritoneal Macrophages
In Vitro Techniques
Molecular Structure
Ultraviolet spectroscopy
Light absorption
Molecular structure
Condensation
Infrared spectroscopy
Spectroscopy

Keywords

  • humic acids
  • macrophages
  • nitrogen oxide
  • peat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide : a Screening Study. / Trofimova, E. S.; Zykova, M. V.; Ligacheva, A. A.; Sherstoboev, E. Yu; Zhdanov, V. V.; Belousov, M. V.; Yusubov, M. S.; Krivoshchekov, S. V.; Danilets, M. G.; Dygai, A. M.

In: Bulletin of Experimental Biology and Medicine, Vol. 161, No. 5, 01.09.2016, p. 687-692.

Research output: Contribution to journalArticle

Trofimova, ES, Zykova, MV, Ligacheva, AA, Sherstoboev, EY, Zhdanov, VV, Belousov, MV, Yusubov, MS, Krivoshchekov, SV, Danilets, MG & Dygai, AM 2016, 'Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide: a Screening Study', Bulletin of Experimental Biology and Medicine, vol. 161, no. 5, pp. 687-692. https://doi.org/10.1007/s10517-016-3486-z
Trofimova, E. S. ; Zykova, M. V. ; Ligacheva, A. A. ; Sherstoboev, E. Yu ; Zhdanov, V. V. ; Belousov, M. V. ; Yusubov, M. S. ; Krivoshchekov, S. V. ; Danilets, M. G. ; Dygai, A. M. / Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide : a Screening Study. In: Bulletin of Experimental Biology and Medicine. 2016 ; Vol. 161, No. 5. pp. 687-692.
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