Effects of dextranation on the pharmacokinetics of short peptides. A PET study on mEGF

Qinghai Zhao, Vladimir Tolmachev, Jörgen Carlsson, Hans Lundqvist, Johanna Sundin, Jan Christer Janson, Anders Sundin

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The effects of dextranation on the biodistribution of mouse epidermal growth factor (mEGF, 6 kDa) were assessed. By reductive amination, mEGF was coupled to 13 and 46 kDa dextran. The two dextranated conjugates and free mEGF were labeled with the positron-emitting nuclide 76Br (T( 1/2 ) = 16 h). After intravenous administration to Sprague Dawley rats, the radioactivity biodistribution was evaluated by positron emission tomography (PET) and by measurements of dissected tissues. The dextranation prolonged the retention time in blood, especially when the dextran chain was long. [76Br]-mEGF-dextran conjugates were shown to have significantly, more than 5 times, lower kidney accumulation than the nonconjugated [76Br]mEGF. In conclusion, dextranation affects the biodistribution of mEGF in vivo giving a prolonged circulation time, a decreased uptake in kidney, and an increased spleen accumulation.

Original languageEnglish
Pages (from-to)938-946
Number of pages9
JournalBioconjugate Chemistry
Volume10
Issue number6
DOIs
Publication statusPublished - Nov 1999
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Effects of dextranation on the pharmacokinetics of short peptides. A PET study on mEGF'. Together they form a unique fingerprint.

Cite this