Effect of the THBS1 Gene Knockout on the Radiation-Induced Cellular Response in a Model System In Vitro

R. R. Savchenko, S. A. Vasilyev, V. S. Fishman, E. S. Sukhikh, L. G. Sukhikh, A. A. Murashkina, I. N. Lebedev

Research output: Contribution to journalArticle

Abstract

Abstract: In this study, the effect of the THBS1 gene knockout on the survival of human tumor cells, the frequency of spontaneous and radiation-induced micronuclei, and the expression profile of genes in the HeLa cell line was investigated. It was shown that the THBS1 gene knockout led to a decrease in the plating efficiency before and after irradiation (1.4-fold, p = 0.0002 and 1.7-fold, p = 0.00009, respectively) and an increase in the frequency of spontaneous and radiation-induced micronuclei (1.9-fold, p = 0.02 and 2.5-fold, p = 0.01, respectively). In addition, expression of genes involved in the DNA repair processes, apoptosis, and G2/M cell cycle checkpoint was changed after THBS1 knockout in comparison with the intact HeLa cell line. Thus, the THBS1 gene knockout leads to an increase in the radiosensitivity of the HeLa cell line. This indicates the possible role of the THBS1 gene in the regulation of a radiation-induced cellular response.

Original languageEnglish
Pages (from-to)618-626
Number of pages9
JournalRussian Journal of Genetics
Volume56
Issue number5
DOIs
Publication statusPublished - 1 May 2020

Keywords

  • CRISPR/Cas9
  • DNA repair processes
  • HeLa
  • radiosensitivity
  • THBS1

ASJC Scopus subject areas

  • Genetics

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    Savchenko, R. R., Vasilyev, S. A., Fishman, V. S., Sukhikh, E. S., Sukhikh, L. G., Murashkina, A. A., & Lebedev, I. N. (2020). Effect of the THBS1 Gene Knockout on the Radiation-Induced Cellular Response in a Model System In Vitro. Russian Journal of Genetics, 56(5), 618-626. https://doi.org/10.1134/S1022795420050129